富含脯氨酸/丝氨酸的螺旋蛋白1减轻小鼠骨髓源性巨噬细胞的焦亡。

IF 3.3 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qiao Wu, Qianqian Wang, Kexin Hu, Tiantian Luo, Jichen Liu, Yazhi Xue, Ling Li, Cuiqi Yang, Rongzhan Lin, Hangyu Pan, Jinhao Wang, Zhigang Guo
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引用次数: 0

摘要

焦亡是一种受调控的炎症细胞死亡过程,在多种疾病中起着重要作用。本研究探讨了富含脯氨酸/丝氨酸的卷曲蛋白1 (PSRC1)在巨噬细胞焦亡和炎症中的作用。本研究报道了PSRC1在热噬细胞中的表达降低,PSRC1的敲除加重了热噬细胞和炎症。PSRC1过表达可减轻巨噬细胞的焦亡和炎症。RNA-seq分析显示,PSRC1调控细胞外基质(ECM)相关基因的表达。具体来说,PSRC1下调了ECM成分periostin (POSTN)的表达。下调POSTN可抑制PSRC1低表达介导的巨噬细胞焦亡。这些结果表明,PSRC1可以通过调节ECM和负性调节POSTN来减轻骨髓源性巨噬细胞(bmdm)的焦亡和炎症。这项研究提供了PSRC1在巨噬细胞焦亡中的作用,并确定了治疗炎症性疾病的潜在靶点。需要进一步的研究在体内和各种疾病模型中证实这些发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Proline/serine-rich coiled-coil protein 1 alleviates pyroptosis in murine bone marrow-derived macrophages.

Pyroptosis is a regulated inflammatory cell death process that plays an essential role in various diseases. This study investigates the role of proline/serine-rich coiled-coil protein 1 (PSRC1) in pyroptosis and inflammation in macrophages. This study reports that PSRC1 expression is decreased in pyroptotic macrophages and that knockout of PSRC1 exacerbates pyroptosis and inflammation. PSRC1 overexpression alleviates pyroptosis and inflammation in macrophages. RNA-seq analysis reveals that PSRC1 regulates the expression of genes involved in the extracellular matrix (ECM). Specifically, PSRC1 downregulates the expression of periostin (POSTN), an ECM component. Knockdown of POSTN suppresses macrophage pyroptosis mediated by low expression of PSRC1. These findings suggest that PSRC1 can alleviate pyroptosis and inflammation in bone marrow-derived macrophages (BMDMs) by regulating the ECM and negatively regulating POSTN. This study provides insights into the role of PSRC1 in macrophage pyroptosis and identifies a potential target for the treatment of inflammatory diseases. Further research is needed to confirm these findings in vivo and in various disease models.

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来源期刊
Acta biochimica et biophysica Sinica
Acta biochimica et biophysica Sinica 生物-生化与分子生物学
CiteScore
5.00
自引率
5.40%
发文量
170
审稿时长
3 months
期刊介绍: Acta Biochimica et Biophysica Sinica (ABBS) is an internationally peer-reviewed journal sponsored by the Shanghai Institute of Biochemistry and Cell Biology (CAS). ABBS aims to publish original research articles and review articles in diverse fields of biochemical research including Protein Science, Nucleic Acids, Molecular Biology, Cell Biology, Biophysics, Immunology, and Signal Transduction, etc.
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