{"title":"基于AuNPs@CdS量子点原位相互作用和FRET机制的hcr方法荧光检测Let-7a miRNA","authors":"Farid Hosseini, Mehdi Dadmehr, Morteza Hosseini","doi":"10.1007/s00604-025-07018-y","DOIUrl":null,"url":null,"abstract":"<div><p>A novel strategy is presented based on integration of nanostructures and molecular amplification was developed for detection of Let-7a miRNA as the carcinogenic cancer marker. Hybridization chain reaction (HCR) was triggered through the presence of Let-7a as the initiator target. This reaction resulted to the formation of AuNPs@CdS QDs assembly due to thiol bonding and following FRET occurred between free AuNPs and CdS QDs. Characterization and morphology of AuNPs@CdS QDs showed in situ accumulation of CdS QDs and afterward optical changes in fluorescence emission. The quenching of fluorescence emission was observed which corresponded to Let-7a concentration in the linear range 10–45 pM with LOD of 2 pM. The sensitivity and selectivity of the performed study confirmed the efficiency of the proposed strategy. Additionally, it demonstrated a high level of specificity and detection performance in real-sample analysis.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fluorescence-based detection of Let-7a miRNA through HCR-based approach upon the in situ interaction of AuNPs@CdS QDs and FRET mechanism\",\"authors\":\"Farid Hosseini, Mehdi Dadmehr, Morteza Hosseini\",\"doi\":\"10.1007/s00604-025-07018-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A novel strategy is presented based on integration of nanostructures and molecular amplification was developed for detection of Let-7a miRNA as the carcinogenic cancer marker. Hybridization chain reaction (HCR) was triggered through the presence of Let-7a as the initiator target. This reaction resulted to the formation of AuNPs@CdS QDs assembly due to thiol bonding and following FRET occurred between free AuNPs and CdS QDs. Characterization and morphology of AuNPs@CdS QDs showed in situ accumulation of CdS QDs and afterward optical changes in fluorescence emission. The quenching of fluorescence emission was observed which corresponded to Let-7a concentration in the linear range 10–45 pM with LOD of 2 pM. The sensitivity and selectivity of the performed study confirmed the efficiency of the proposed strategy. Additionally, it demonstrated a high level of specificity and detection performance in real-sample analysis.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 3\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-02-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07018-y\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07018-y","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Fluorescence-based detection of Let-7a miRNA through HCR-based approach upon the in situ interaction of AuNPs@CdS QDs and FRET mechanism
A novel strategy is presented based on integration of nanostructures and molecular amplification was developed for detection of Let-7a miRNA as the carcinogenic cancer marker. Hybridization chain reaction (HCR) was triggered through the presence of Let-7a as the initiator target. This reaction resulted to the formation of AuNPs@CdS QDs assembly due to thiol bonding and following FRET occurred between free AuNPs and CdS QDs. Characterization and morphology of AuNPs@CdS QDs showed in situ accumulation of CdS QDs and afterward optical changes in fluorescence emission. The quenching of fluorescence emission was observed which corresponded to Let-7a concentration in the linear range 10–45 pM with LOD of 2 pM. The sensitivity and selectivity of the performed study confirmed the efficiency of the proposed strategy. Additionally, it demonstrated a high level of specificity and detection performance in real-sample analysis.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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