四康唑和戊康唑对大水蚤的慢性毒性：生长、繁殖和基因表达变化的观察

IF 8 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Science of the Total Environment Pub Date : 2025-02-12 DOI:10.1016/j.scitotenv.2025.178774

Ozkan Aksakal , Hatice Dane , Cihan Gur , Turgay Sisman

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引用次数: 0

摘要

四康唑和戊康唑是广泛使用的杀菌剂，属于康唑族。由于这些杀菌剂的使用越来越多，它们在水生环境中的浓度不断增加，对水生生物构成严重威胁。然而，尚未有研究调查四康唑和戊康唑对水生无脊椎动物的影响。本研究首次检测了不同浓度四康唑和戊康唑处理21 d后D. magna的生长、繁殖、存活率以及解毒相关基因（HR96、P-GP、CYP360A8、GST）和繁殖相关基因（CUT、CYP314、DMRT、VTG）表达的变化。四康唑48h EC50值为12.35 μg/L，戊康唑为326.8 μg/L。慢性毒性试验结果表明，不同浓度的四康唑和戊康唑使马格纳家鼠体长、每只雌鼠总子代数、换羽频率、每分钟心跳率和成活率降低，并使马格纳家鼠首窝日数增加。解毒和繁殖相关基因的表达随杀菌剂种类和浓度的不同而变化。总的来说，与解毒有关的基因转录受到杀菌剂的影响更大。结果表明，四康唑和戊康唑均通过抑制大鼠的生长繁殖和影响解毒途径而对大鼠产生毒性。

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Chronic toxicity of tetraconazole and penconazole to Daphnia magna: Insights of growth, reproduction and gene expression changes

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Chronic toxicity of tetraconazole and penconazole to Daphnia magna: Insights of growth, reproduction and gene expression changes

Tetraconazole and penconazole are widely used fungicides belonging to the conazole family. Due to the increasing use of these fungicides, their concentrations in aquatic environments are increasing and imply a serious threat to aquatic organisms. However, no studies have investigated the effects of tetraconazole and penconazole on aquatic invertebrates. This study examined for the first time, changes in growth, reproduction, and survival rate as well as changes in the expression of genes related to detoxification (HR96, P-GP, CYP360A8, GST) and reproduction (CUT, CYP314, DMRT, VTG) in D. magna after exposed to different tetraconazole and penconazole concentrations for 21 days. The 48-h EC₅₀ value was 12.35 μg/L for tetraconazole and 326.8 μg/L for penconazole. Chronic toxicity results showed that exposure to varying concentrations of tetraconazole and penconazole decreased body length, total offspring per female, molting frequency, heartbeat rate per minute, and survival rate, and increased day to the first brood in D. magna. The expression of genes related to detoxification and reproduction changed depending on the fungicide type and concentration. In general, transcription of genes related to detoxification was more affected by fungicides. The results revealed that tetraconazole and penconazole caused toxicity in D. magna by inhibiting growth and reproduction and affecting detoxification pathways similarly.

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来源期刊

Science of the Total Environment 环境科学-环境科学

CiteScore

17.60

自引率

10.20%

发文量

8726

审稿时长

2.4 months

期刊介绍： The Science of the Total Environment is an international journal dedicated to scientific research on the environment and its interaction with humanity. It covers a wide range of disciplines and seeks to publish innovative, hypothesis-driven, and impactful research that explores the entire environment, including the atmosphere, lithosphere, hydrosphere, biosphere, and anthroposphere. The journal's updated Aims & Scope emphasizes the importance of interdisciplinary environmental research with broad impact. Priority is given to studies that advance fundamental understanding and explore the interconnectedness of multiple environmental spheres. Field studies are preferred, while laboratory experiments must demonstrate significant methodological advancements or mechanistic insights with direct relevance to the environment.