Xue Wang, Xiangqi Shao, Liang Yu, Jianru Sun, Xiang-Sha Yin, Zhen Chen, Yuanyuan Xu, Naili Wang, Di Zhang, Wenying Qiu, Fan Liu, Chao Ma
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引用次数: 0
摘要
中枢神经系统的微环境非常复杂，在维持神经元功能方面起着至关重要的作用，而神经元功能的维持影响着阿尔茨海默病（AD）的进展。脑pH值是脑微环境在调节各种生理过程中的一个重要方面。然而，这一机制的具体机制和作用尚不完全清楚。为了更好地了解脑pH值与AD之间的关系，我们分析了来自国家人脑发育和功能银行的368名捐赠者的死后脑样本的额叶脑pH值和AD病理评分，其中96人被诊断为AD病理。分析显示，AD患者脑pH值显著下降，这与β-淀粉样蛋白斑块和磷酸化tau蛋白密切相关。本研究阐明了对照组与AD组cd68阳性小胶质细胞蛋白表达水平的差异（t = 3.198, df = 20, P = 0.0045），其蛋白表达水平与脑pH值呈负相关（F = 26.93, P = 0.0006）。我们的研究结果表明，AD患者病理脑组织中cd68阳性小胶质细胞激活增加和溶酶体稳态破坏可能导致脑pH降低。
Changes in the pH value of the human brain in Alzheimer's disease pathology correlated with CD68-positive microglia: a community-based autopsy study in Beijing, China.
The microenvironment of the central nervous system is highly complex and plays a crucial role in maintaining the function of neurons, which influences Alzheimer's disease (AD) progression. The pH value of the brain is a critical aspect of the brain microenvironment in regulating various physiological processes. However, the specific mechanisms and role of this mechanism are not yet fully understood. To better understand the relationship between brain pH and AD, we analyzed the brain pH of the frontal lobe and AD pathology scores in postmortem brain samples from 368 donors from the National Human Brain Bank for Development and Function, 96 of whom were diagnosed with AD pathology. Analysis revealed a significant decrease in brain pH in AD patients, which was strongly correlated with β-amyloid plaques and phosphorylated tau proteins. Here, we elucidated the differential protein expression level of CD68-positive microglia between control and AD groups (t = 3.198, df = 20, P = 0.0045), and its protein expression level was correlated negatively with the brain pH value (F = 26.93, p = 0.0006). Our findings revealed that increased activation of CD68-positive microglia and disrupted lysosomal homeostasis in the pathological brain tissue of individuals with AD may lead to a decrease in brain pH.
期刊介绍：
Molecular Brain is an open access, peer-reviewed journal that considers manuscripts on all aspects of studies on the nervous system at the molecular, cellular, and systems level providing a forum for scientists to communicate their findings.
Molecular brain research is a rapidly expanding research field in which integrative approaches at the genetic, molecular, cellular and synaptic levels yield key information about the physiological and pathological brain. These studies involve the use of a wide range of modern techniques in molecular biology, genomics, proteomics, imaging and electrophysiology.
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