利用正交池法在斑马鱼中发现恢复视力的新药。

4区医学 Q2 Biochemistry, Genetics and Molecular Biology

Advances in experimental medicine and biology Pub Date : 2025-01-01 DOI:10.1007/978-3-031-76550-6_80

Justine O'Brien, Patrizia Colucci, Yolanda Alvarez, Breandán N Kennedy

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引用次数: 0

摘要

遗传性视网膜变性（IRDs）和年龄相关性黄斑变性（AMD）的光感受器和视网膜色素上皮（RPE）功能障碍需要创新的治疗方法来保护视力。视力障碍给全球带来了沉重的经济负担，据世界卫生组织报告，每年全球生产力损失约为4 110亿美元。目前的治疗方法是有限的，强调了新的解决方案的紧迫性。利用新的筛选技术，可以发现恢复视力的新药。本文描述了一个工作流程，利用正交池筛选随机文库化合物，以恢复视力和评估遗传性失明atp6v0e1-/-斑马鱼模型中的光动力学反应（OKR）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Uncovering Novel Drugs that Restore Vision Using Orthogonal Pooling in Zebrafish.

Photoreceptor and retinal pigment epithelium (RPE) dysfunction in inherited retinal degenerations (IRDs) and age-related macular degeneration (AMD) necessitate innovative therapies to preserve vision. Vision impairment incurs a substantial global economic burden, with the World Health Organization reporting an annual global productivity loss of approximately $411 billion. Current treatments are limited, underscoring the urgency for novel solutions. Leveraging new screening techniques, novel drugs restoring vision can be uncovered. Here, a workflow is described utilising orthogonal pooling to screen randomised library compounds for drug hits restoring vision and assessing the optokinetic response (OKR) in the atp6v0e1^-/- zebrafish model of inherited blindness.

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来源期刊

Advances in experimental medicine and biology 医学-医学：研究与实验

CiteScore

5.90

自引率

0.00%

发文量

465

审稿时长

2-4 weeks

期刊介绍： Advances in Experimental Medicine and Biology provides a platform for scientific contributions in the main disciplines of the biomedicine and the life sciences. This series publishes thematic volumes on contemporary research in the areas of microbiology, immunology, neurosciences, biochemistry, biomedical engineering, genetics, physiology, and cancer research. Covering emerging topics and techniques in basic and clinical science, it brings together clinicians and researchers from various fields.