Wistar大鼠伏隔核胆碱能中间神经元消融后酒精消耗减少

IF 2.6 3区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Anna Loftén, Davide Cadeddu, Klara Danielsson, Rosita Stomberg, Louise Adermark, Bo Söderpalm, Mia Ericson
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引用次数: 0

摘要

酒精使用障碍是一种严重的精神健康状况,会导致医疗后果和过早死亡。酒精激活中边缘多巴胺系统,导致伏隔核细胞外多巴胺(DA)增加,这一事件与酒精的强化作用有关。胆碱能中间神经元(CIN)是伏隔区DA信号的重要调节剂,伏隔区CIN的消耗减弱了酒精诱导的细胞外DA的增加。本研究的目的是探讨伏隔区CIN在酒精相关行为中的功能作用。为此,将抗胆碱乙酰转移酶皂苷双侧局部注入雄性Wistar大鼠伏隔核诱导CIN消融。在消融和假药治疗的大鼠酒精消耗研究使用两瓶选择间歇性饮酒范式。与假药对照组相比,CIN缺失的大鼠消耗的酒精明显减少。治疗组之间在蔗糖偏好、运动活动、水摄入量或体重增加方面没有差异,这表明消融术选择性地影响了酒精相关行为。总之,本研究进一步支持伏隔区CIN在调节酒精消耗行为中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Reduced Alcohol Consumption Following Ablation of Cholinergic Interneurons in the Nucleus Accumbens of Wistar Rats

Reduced Alcohol Consumption Following Ablation of Cholinergic Interneurons in the Nucleus Accumbens of Wistar Rats

Alcohol use disorder is a severe mental health condition causing medical consequences and preterm death. Alcohol activates the mesolimbic dopamine system leading to an increase of extracellular dopamine (DA) in the nucleus accumbens, an event that is associated with the reinforcing effects of alcohol. Cholinergic interneurons (CIN) are important modulators of accumbal DA signalling, and depletion of accumbal CIN attenuates the alcohol-induced increase in extracellular DA. The aim of this study was to explore the functional role of accumbal CIN in alcohol-related behaviour. To this end, ablation of CIN was induced by local administration of anticholine acetyltransferase-saporin bilaterally into the nucleus accumbens of male Wistar rats. Alcohol consumption in ablated and sham-treated rats was studied using a two-bottle-choice intermittent alcohol consumption paradigm. Rats with depleted CIN consumed significantly less alcohol than sham-treated controls. No differences in sucrose preference, motor activity, water intake or weight gain were noted between treatment groups, suggesting that the ablation selectively affected alcohol-related behaviour. In conclusion, this study further supports a role for accumbal CIN in regulating alcohol-consummatory behaviour.

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来源期刊
Addiction Biology
Addiction Biology 生物-生化与分子生物学
CiteScore
8.10
自引率
2.90%
发文量
118
审稿时长
6-12 weeks
期刊介绍: Addiction Biology is focused on neuroscience contributions and it aims to advance our understanding of the action of drugs of abuse and addictive processes. Papers are accepted in both animal experimentation or clinical research. The content is geared towards behavioral, molecular, genetic, biochemical, neuro-biological and pharmacology aspects of these fields. Addiction Biology includes peer-reviewed original research reports and reviews. Addiction Biology is published on behalf of the Society for the Study of Addiction to Alcohol and other Drugs (SSA). Members of the Society for the Study of Addiction receive the Journal as part of their annual membership subscription.
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