Sameera Sh. Mohammed Ameen, Faisal Algethami, Khalid M. Omer
{"title":"松针衍生的氧化酶样锰纳米酶：可持续纳米酶，可扩展合成，以及亚硝酸盐的视觉和比色检测","authors":"Sameera Sh. Mohammed Ameen, Faisal Algethami, Khalid M. Omer","doi":"10.1007/s00604-025-07024-0","DOIUrl":null,"url":null,"abstract":"<div><p> Manganese nanoparticles (Mn NPs) were successfully synthesized using a cost-effective and eco-friendly biogenic approach, with <i>pinus brutia</i> pine needles<i>.</i> The produced Mn NPs were thoroughly characterized using common spectroscopic and microscopic techniques. The Mn NPs exhibited remarkable oxidase-like activity, effectively catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its oxidized form (oxTMB) over a broad temperature range. This robust catalytic performance highlights their versatility and stability under varying thermal conditions, making them suitable for diverse applications. Nitrite forms orange-yellow color via diazotization of the blue oxTMB. An image processing algorithm analyzes the image changes induced by addition of nitrite. Under optimized conditions, the visual-based platform demonstrated a broad linear response for NO₂⁻ detection, spanning 0.0–58.0 uM and the detection limit was 0.11 μM. The visual method demonstrated exceptional sensitivity and recovery for nitrite detection in food samples. Combining simplicity, cost-effectiveness, and eco-friendliness, this instrument-free approach ensures accurate, portable, and point-of-care nitrite detection, ideal for real-world food testing applications.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pine needle-derived oxidase-like Mn nanozymes: sustainable nanozyme, scalable synthesis, and visual and colorimetric nitrite detection\",\"authors\":\"Sameera Sh. Mohammed Ameen, Faisal Algethami, Khalid M. Omer\",\"doi\":\"10.1007/s00604-025-07024-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p> Manganese nanoparticles (Mn NPs) were successfully synthesized using a cost-effective and eco-friendly biogenic approach, with <i>pinus brutia</i> pine needles<i>.</i> The produced Mn NPs were thoroughly characterized using common spectroscopic and microscopic techniques. The Mn NPs exhibited remarkable oxidase-like activity, effectively catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its oxidized form (oxTMB) over a broad temperature range. This robust catalytic performance highlights their versatility and stability under varying thermal conditions, making them suitable for diverse applications. Nitrite forms orange-yellow color via diazotization of the blue oxTMB. An image processing algorithm analyzes the image changes induced by addition of nitrite. Under optimized conditions, the visual-based platform demonstrated a broad linear response for NO₂⁻ detection, spanning 0.0–58.0 uM and the detection limit was 0.11 μM. The visual method demonstrated exceptional sensitivity and recovery for nitrite detection in food samples. Combining simplicity, cost-effectiveness, and eco-friendliness, this instrument-free approach ensures accurate, portable, and point-of-care nitrite detection, ideal for real-world food testing applications.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 3\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-02-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07024-0\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07024-0","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Pine needle-derived oxidase-like Mn nanozymes: sustainable nanozyme, scalable synthesis, and visual and colorimetric nitrite detection
Manganese nanoparticles (Mn NPs) were successfully synthesized using a cost-effective and eco-friendly biogenic approach, with pinus brutia pine needles. The produced Mn NPs were thoroughly characterized using common spectroscopic and microscopic techniques. The Mn NPs exhibited remarkable oxidase-like activity, effectively catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its oxidized form (oxTMB) over a broad temperature range. This robust catalytic performance highlights their versatility and stability under varying thermal conditions, making them suitable for diverse applications. Nitrite forms orange-yellow color via diazotization of the blue oxTMB. An image processing algorithm analyzes the image changes induced by addition of nitrite. Under optimized conditions, the visual-based platform demonstrated a broad linear response for NO₂⁻ detection, spanning 0.0–58.0 uM and the detection limit was 0.11 μM. The visual method demonstrated exceptional sensitivity and recovery for nitrite detection in food samples. Combining simplicity, cost-effectiveness, and eco-friendliness, this instrument-free approach ensures accurate, portable, and point-of-care nitrite detection, ideal for real-world food testing applications.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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