小麦中osca的分离和TaOSCA14D的过表达提高了其耐盐性

IF 4 3区 生物学 Q1 PLANT SCIENCES
Ruiping Su, Yuning Wang, Ping Cui, Geng Tian, Yuxiang Qin
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引用次数: 0

摘要

盐胁迫是限制植物生长和生产力的主要环境因素。在盐胁迫早期,细胞内Ca2+浓度升高,从而触发渗透胁迫耐受信号通路。OSCAs编码高渗透门控钙通道,并在拟南芥中起渗透传感器的作用。但OSCAs在小麦抗盐胁迫中的作用尚未阐明。在这项研究中,我们鉴定了42个TaOSCAs,并检测了它们在12个组织和盐胁迫下的表达模式。此外,选择盐诱导的TaOSCA14D进行盐胁迫响应功能研究。TaOSCA14D分别受NaCl、PEG、外源ABA的诱导。TaOSCA14D在拟南芥和小麦中的过表达增加了盐胁迫的耐受性。盐胁迫相关标记基因SnRK2s、ABFs、RD29B在TaOSCA14D转基因植株中的表达量高于野生型。此外,转基因植株的可溶性糖和脯氨酸含量高于野生型植株。过表达TaOSCA14D促进了开花,降低了穗长和穗粒数。这些结果对osca在小麦耐盐胁迫中的作用及其在农艺性状中的作用有一定的启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation of OSCAs in wheat and over-expression of TaOSCA14D increased salt stress tolerance
Salt stress is a major environmental factor that limits plant growth and productivity. In the early stage of salt stress, the intracellular Ca2+ concentration elevates, thereby triggering osmotic stress tolerance signaling pathway. OSCAs encode hyperosmotic gated calcium channels and function as osmotic sensors in Arabidopsis. But the functions of OSCAs in wheat responding to salt stress have not been elucidated. In this study, we identified 42 TaOSCAs and examined their expression pattern in 12 tissues and under salt stress. Further, the salt inducible TaOSCA14D was selected for functional study in response to salt stress. TaOSCA14D was induced by NaCl, PEG, exogenous ABA treatment. Over-expression of TaOSCA14D in Arabidopsis and wheat increased salt stress tolerance. Salt stress related marker genes SnRK2s, ABFs, RD29B were higher expressed in TaOSCA14D transgenic plants than in the wild type under NaCl treatment. Furthermore, the soluble sugar and proline content were higher in transgenic plants than in wild-type ones. Over-expression of TaOSCA14D promoted flowering, decreased spike length and the grain number of per spike. All these results shed some light on the function of OSCAs in tolerance to salt stress and its roles in agronomic trait in wheat.
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来源期刊
Journal of plant physiology
Journal of plant physiology 生物-植物科学
CiteScore
7.20
自引率
4.70%
发文量
196
审稿时长
32 days
期刊介绍: The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.
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