二氧化碳通过刺激脂肪酸代谢和降低血小板葡萄糖消耗来减轻血小板储存损伤

IF 3.4 3区医学 Q2 HEMATOLOGY

Research and Practice in Thrombosis and Haemostasis Pub Date : 2025-01-01 DOI:10.1016/j.rpth.2025.102681

Shunli Gu, Jinmei Xu, Erxiong Liu, Xuejia Hou, Ning An, Yaozhen Chen, Zhixin Liu, Wenting Wang, Xingbin Hu, Wen Yin

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引用次数: 0

摘要

及时输注血小板对患者的生存至关重要，临床对血小板输注的需求一直在稳步增长。然而，在体外保存过程中发生的血小板储存病变（psl）加剧了这些不足。PSL受各种因素的显著影响，包括温度、气体成分和缓冲系统。近年来，人们积极探索缓解PSLs和改善血小板储存的策略。目的本研究旨在探讨升高的二氧化碳（CO2）水平是否能改善血小板在储存过程中的质量和功能。方法28例献血者的血小板浓缩液在22±2°C对照或3% CO2条件下保存7 d。通过扫描电镜、粘附、聚集、凝块收缩、活化、细胞凋亡、血气、三磷酸腺苷、代谢组学分析以及体内血栓形成和生存试验来评估血小板质量。结果增加储存环境中的CO2浓度可减轻血小板的PSLs，提高血小板的质量。结论本研究强调了利用高CO2储存环境改善血小板保存的潜在益处，为解决临床血小板短缺提供了一种有希望的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Carbon dioxide alleviates platelet storage lesions via stimulating fatty acid metabolism and reducing platelet glucose consumption

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Carbon dioxide alleviates platelet storage lesions via stimulating fatty acid metabolism and reducing platelet glucose consumption

Background

The timely administration of platelet transfusions is critical for patient survival, and the clinical demand for platelet transfusions has been steadily increasing. However, platelet storage lesions (PSLs) that develop during in vitro preservation exacerbate these shortages. The PSL is significantly influenced by various factors, including temperature, gas composition, and buffering systems. Strategies to mitigate PSLs and improve platelet storage have been actively explored in recent years.

Objectives

This study aimed to investigate whether elevated carbon dioxide (CO₂) levels improve platelet quality and functionality during storage.

Methods

Platelet concentrates from 28 donors were stored under control or 3% CO₂ conditions at 22 ± 2 °C for up to 7 days. Platelet quality was evaluated through scanning electron microscopy, adhesion, aggregation, clot contraction, activation, apoptosis assays, blood gas, adenosine triphosphate, metabolomics analyses, and in vivo thrombosis and survival tests.

Results

Our findings indicate that increasing the CO₂ concentration in the storage environment mitigates PSLs and improves platelet quality.

Conclusion

Our study highlights the potential benefits of utilizing a high CO₂ storage environment to improve platelet preservation, offering a promising method to address clinical platelet shortages.

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