阴囊热应激和己酮茶碱处理对种马睾丸激素产生及其受体基因表达的影响

IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES
Y.F.R. Sancler-Silva , E.R. Silva-Junior , A. Esteller-Vico , Y.L. Boakari , H. El-Sheikh Ali , F.O. Papa , B.A. Ball
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引用次数: 0

摘要

根据阴囊热应激的持续时间和强度,对睾丸实质不同细胞的影响程度各不相同,最初损害精子发生,在更严重的情况下,损害激素的产生。己酮茶碱已被证明可以减少马睾丸退化造成的组织学损伤,但其对热应激后睾丸激素产生的影响尚不清楚。本研究评估了热应激和己酮可可碱处理对阴囊热应激的种马睾丸活检中血清睾酮、硫酸雌酮和抗勒氏激素(AMH)浓度的影响,以及它们受体基因表达的影响。将14匹马分为3组:对照组(CRL, n=4)、睾丸变性组(DEG, n=5)和睾丸变性组(DEG+PTX, n=5)。用50°C充满空气的保温袋将阴囊保温1小时,每天2次(清晨和下午晚些时候),连续2天(D-1和D0),诱导睾丸变性。从第二天(D1)开始,每12小时口服己酮茶碱(17 mg/kg),连续30天。每周1次颈外静脉穿刺采血,连续8周。采用酶联免疫吸附法测定血清睾酮、AMH、硫酸雌酮浓度。在第30天(D30)和第60天(D60),取睾丸活检并快速冷冻,以备后期RT-qPCR评估。评估的基因为AR、ESR1、ESR2和AMH。正态分布数据采用混合模型方差分析,非正态分布数据采用Wilcoxon检验,采用JMP 12.0软件(SAS Institute, Cary, NC, US)。在60天的评估期内,试验组间血清睾酮、硫酸雌酮和AMH浓度均无显著差异,且均与健康动物的参考值一致。但热损伤60 d后,己酮可可碱处理组AMH基因表达量较对照组显著升高(P<0.05)。其他被评估的基因在两组之间没有观察到其他差异。综上所述,本研究诱导的热应激不影响睾丸中性激素的产生或激素受体的基因表达。此外,在治疗60天后,己酮茶碱似乎对AMH基因的上调起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Testicular hormones production and the gene expression of their receptors in the testicles of stallions subjected to scrotal heat stress and treated with pentoxifylline
Depending on the duration and intensity of scrotal heat stress, the impact on different cells of the testicular parenchyma varies in severity, initially compromising spermatogenesis and, in more severe cases, impairing hormonal production. Pentoxifylline has been shown to reduce histological damage from testicular degeneration in horses, but its impact on testicular hormonal production after heat stress is still unknown. This study evaluated the effects of heat stress and pentoxifylline treatment on serum concentrations of testosterone, estrone sulfate, and anti-Müllerian hormone (AMH), as well as on the gene expression of their receptors in testicular biopsies of stallions subjected to scrotal heat stress. A total of 14 stallions were divided into three groups: Control (CRL, n=4), Testicular Degeneration (DEG, n=5), and Testicular Degeneration Treated with Pentoxifylline (DEG+PTX, n=5). Testicular degeneration was induced by scrotal insulation using a thermal bag filled with air at 50°C for 1 hour, twice a day (early morning and late afternoon), over two consecutive days (D-1 and D0). From the following day (D1), oral pentoxifylline (17 mg/kg) was administered every 12 hours for 30 days. Blood samples were collected by external jugular venipuncture once a week for eight consecutive weeks. Serum concentrations of testosterone, AMH, and estrone sulfate were determined by ELISA. On days 30 (D30) and 60 (D60), testicular biopsies were taken and snap-frozen for later evaluation by RT-qPCR. The genes evaluated were AR, ESR1, ESR2, and AMH. Normally distributed data were analyzed using a mixed-model ANOVA, and non-normally distributed data were analyzed using the Wilcoxon test, using JMP 12.0 software (SAS Institute, Cary, NC, US). No significant differences were observed between the experimental groups in serum concentrations of testosterone, estrone sulfate, and AMH over the 60-day evaluation period, and all values were consistent with reference values for healthy animals. However, after 60 days of thermal injury, there was a significant increase in AMH gene expression in the pentoxifylline-treated group compared to the control group (P<0.05). No other differences were observed between groups for the other genes evaluated. In conclusion, the heat stress induced in this study did not impact gonadal hormone production or the gene expression of hormonal receptors in the testes. Furthermore, pentoxifylline appears to exert an effect on the upregulation of the AMH gene after 60 days of treatment.
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来源期刊
Journal of Equine Veterinary Science
Journal of Equine Veterinary Science 农林科学-兽医学
CiteScore
2.70
自引率
7.70%
发文量
249
审稿时长
77 days
期刊介绍: Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.
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