AhR通过上调SERPINB2导致变应性鼻炎的促炎细胞因子和趋化因子的释放和气道上皮屏障功能障碍。

IF 2.5 4区 医学 Q3 ALLERGY
Xinhong Lu, Gang Gao, Jiaxin Jin, Xiaobo Huang, Liwei Lin, Maohua Qian, Nimei Shen
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引用次数: 0

摘要

变应性鼻炎(Allergic rhinitis, AR)是一种鼻腔过敏疾病,在全球范围内的发病率急剧上升。纤溶酶原激活物抑制剂2型(SERPINB2)是一种炎症介质,也可以被芳烃受体(AhR)激活,芳烃受体是一种调节对环境刺激反应的核受体。这项工作致力于研究SERPINB2在AR中的作用及其与AhR的关系。方法采用酶联免疫吸附法(ELISA)测定经白细胞介素-4 (IL-4)或组胺刺激的人鼻上皮细胞(HNEpCs)的促炎因子和趋化因子。上皮屏障功能通过上皮电阻值(TER)评估。Western blot和免疫荧光染色评价紧密连接蛋白。RT-qPCR和Western blot检测SERPINB2和AhR的表达。结果IL-4和组胺诱导的HNEpCs促炎细胞因子和趋化因子释放加重,TER值降低,紧密连接蛋白表达下调。在IL-4或组胺处理的HNEpCs中,SERPINB2和AhR均过表达,SERPINB2缺失减轻了炎症反应并保留了上皮屏障功能。此外,AhR敲低降低SERPINB2表达,SERPINB2上调逆转了AhR沉默在HNEpCs对IL-4或组胺的炎症反应和上皮屏障功能障碍中的保护作用。结论AhR可能激活SERPINB2,加重AR的炎症和气道上皮屏障功能障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
AhR Causes the Release of Proinflammatory Cytokines and Chemokines and Airway Epithelial Barrier Dysfunction in Allergic Rhinitis via Upregulating SERPINB2.

Introduction: Allergic rhinitis (AR) remains a kind of nasal hypersensitivity illness with a considerable surge in incidence across the globe. Plasminogen activator inhibitor type 2 (SERPINB2) is an inflammatory mediator that can also be activated by aryl hydrocarbon receptor (AhR), a nuclear receptor that modulates the response to environmental stimuli. This work was devoted to the investigation into the role of SERPINB2 in AR and its relationship with AhR.

Methods: In human nasal epithelial cells (HNEpCs) stimulated with interleukin-4 (IL-4) or histamine, an enzyme-linked immunosorbent assay was adopted for quantifying proinflammatory cytokines and chemokines. Epithelial barrier function was assessed by transepithelial electrical resistance (TER). Western blot and immunofluorescence staining evaluated tight junction proteins. Reverse transcription-quantitative PCR and Western blot determined SERPINB2 and AhR expressions.

Results: The release of proinflammatory cytokines and chemokines was aggravated, TER value was reduced and the expressions of tight junction proteins were downregulated in HNEpCs when induced by IL-4 or histamine. SERPINB2 and AhR were both overexpressed and SERPINB2 depletion relieved inflammatory response and preserved epithelial barrier function in IL-4- or histamine-treated HNEpCs. Besides, AhR knockdown decreased SERPINB2 expression and SERPINB2 upregulation reverted the protective role of AhR silencing in the inflammatory response and epithelial barrier dysfunction in HNEpCs in response to IL-4 or histamine.

Conclusion: Collectively, AhR might activate SERPINB2 to exacerbate inflammation and airway epithelial barrier dysfunction in AR.

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来源期刊
CiteScore
5.60
自引率
3.60%
发文量
105
审稿时长
2 months
期刊介绍: ''International Archives of Allergy and Immunology'' provides a forum for basic and clinical research in modern molecular and cellular allergology and immunology. Appearing monthly, the journal publishes original work in the fields of allergy, immunopathology, immunogenetics, immunopharmacology, immunoendocrinology, tumor immunology, mucosal immunity, transplantation and immunology of infectious and connective tissue diseases.
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