{"title":"热休克预处理骨髓间充质干细胞促进糖尿病足溃疡大鼠模型伤口愈合。","authors":"Xi Lin, Qi Lin","doi":"10.1111/dme.15507","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Diabetic foot ulcers (DFUs) are the severe chronic complications of diabetes, amputation is required when ulcers cause severe loss of tissue or evoke a life-threatening infection. Mesenchymal stem cells (MSCs) have shown a good effect in helping DFU healing, though the efficiency needs to be improved. This study aimed to investigate the effects of heat shock pretreatment on the improvement of the therapeutic effects of MSCs.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Primary rat bone marrow MSCs (BMSCs) were isolated and stimulated with heat shock pretreatment and then tested on a DFU rat model. Alkaline phosphatase, Alizarin Red S, and Oil Red O were stained to check the osteogenic differentiation ability of heat shock-pretreated BMSCs. The effect of heat shock pretreatment on the inflammatory response of macrophages was studied with the lipopolysaccharides stimulation model on a mouse macrophage cell line RAW264.7. The impact of heat shock-pretreated BMSCs on dermal fibroblasts was also checked. Last, heat shock-pretreated BMSCs were tested on a DFU rat model.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Heat shock-pretreated BMSCs were characterized by the expression of CD105 and CD44. Heat shock pre-stimulation did not affect cell viability when cultured up to 96 h. Heat shock pre-stimulated BMSCs inhibited the inflammatory response by reducing the pro-inflammatory cytokine production (IL-1β, IL-6, and TNF-α) and enhancing the anti-inflammatory cytokine production (IL-10) (at least all <i>p</i> < 0.01), as well as increasing the ratio of M2 polarization macrophages to M1 polarization in vitro (<i>p</i> < 0.001). Heat shock pre-stimulated BMSCs enhanced the growth and migration of dermal fibroblasts in vitro (<i>p</i> < 0.001). Heat shock-BMSCs promoted the M2 polarization level of macrophages in wound tissues in a DFU rat model.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Heat shock pretreatment could enhance the therapeutic effect of BMSCs on wound healing in a DFU rat model.</p>\n </section>\n </div>","PeriodicalId":11251,"journal":{"name":"Diabetic Medicine","volume":"42 5","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Heat shock-pretreated bone marrow mesenchymal stem cells accelerate wound healing in a diabetic foot ulcer rat model\",\"authors\":\"Xi Lin, Qi Lin\",\"doi\":\"10.1111/dme.15507\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Diabetic foot ulcers (DFUs) are the severe chronic complications of diabetes, amputation is required when ulcers cause severe loss of tissue or evoke a life-threatening infection. Mesenchymal stem cells (MSCs) have shown a good effect in helping DFU healing, though the efficiency needs to be improved. This study aimed to investigate the effects of heat shock pretreatment on the improvement of the therapeutic effects of MSCs.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Primary rat bone marrow MSCs (BMSCs) were isolated and stimulated with heat shock pretreatment and then tested on a DFU rat model. Alkaline phosphatase, Alizarin Red S, and Oil Red O were stained to check the osteogenic differentiation ability of heat shock-pretreated BMSCs. The effect of heat shock pretreatment on the inflammatory response of macrophages was studied with the lipopolysaccharides stimulation model on a mouse macrophage cell line RAW264.7. The impact of heat shock-pretreated BMSCs on dermal fibroblasts was also checked. Last, heat shock-pretreated BMSCs were tested on a DFU rat model.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Heat shock-pretreated BMSCs were characterized by the expression of CD105 and CD44. Heat shock pre-stimulation did not affect cell viability when cultured up to 96 h. Heat shock pre-stimulated BMSCs inhibited the inflammatory response by reducing the pro-inflammatory cytokine production (IL-1β, IL-6, and TNF-α) and enhancing the anti-inflammatory cytokine production (IL-10) (at least all <i>p</i> < 0.01), as well as increasing the ratio of M2 polarization macrophages to M1 polarization in vitro (<i>p</i> < 0.001). Heat shock pre-stimulated BMSCs enhanced the growth and migration of dermal fibroblasts in vitro (<i>p</i> < 0.001). Heat shock-BMSCs promoted the M2 polarization level of macrophages in wound tissues in a DFU rat model.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Heat shock pretreatment could enhance the therapeutic effect of BMSCs on wound healing in a DFU rat model.</p>\\n </section>\\n </div>\",\"PeriodicalId\":11251,\"journal\":{\"name\":\"Diabetic Medicine\",\"volume\":\"42 5\",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-02-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diabetic Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/dme.15507\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diabetic Medicine","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/dme.15507","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
Heat shock-pretreated bone marrow mesenchymal stem cells accelerate wound healing in a diabetic foot ulcer rat model
Background
Diabetic foot ulcers (DFUs) are the severe chronic complications of diabetes, amputation is required when ulcers cause severe loss of tissue or evoke a life-threatening infection. Mesenchymal stem cells (MSCs) have shown a good effect in helping DFU healing, though the efficiency needs to be improved. This study aimed to investigate the effects of heat shock pretreatment on the improvement of the therapeutic effects of MSCs.
Methods
Primary rat bone marrow MSCs (BMSCs) were isolated and stimulated with heat shock pretreatment and then tested on a DFU rat model. Alkaline phosphatase, Alizarin Red S, and Oil Red O were stained to check the osteogenic differentiation ability of heat shock-pretreated BMSCs. The effect of heat shock pretreatment on the inflammatory response of macrophages was studied with the lipopolysaccharides stimulation model on a mouse macrophage cell line RAW264.7. The impact of heat shock-pretreated BMSCs on dermal fibroblasts was also checked. Last, heat shock-pretreated BMSCs were tested on a DFU rat model.
Results
Heat shock-pretreated BMSCs were characterized by the expression of CD105 and CD44. Heat shock pre-stimulation did not affect cell viability when cultured up to 96 h. Heat shock pre-stimulated BMSCs inhibited the inflammatory response by reducing the pro-inflammatory cytokine production (IL-1β, IL-6, and TNF-α) and enhancing the anti-inflammatory cytokine production (IL-10) (at least all p < 0.01), as well as increasing the ratio of M2 polarization macrophages to M1 polarization in vitro (p < 0.001). Heat shock pre-stimulated BMSCs enhanced the growth and migration of dermal fibroblasts in vitro (p < 0.001). Heat shock-BMSCs promoted the M2 polarization level of macrophages in wound tissues in a DFU rat model.
Conclusion
Heat shock pretreatment could enhance the therapeutic effect of BMSCs on wound healing in a DFU rat model.
期刊介绍：
Diabetic Medicine, the official journal of Diabetes UK, is published monthly simultaneously, in print and online editions.
The journal publishes a range of key information on all clinical aspects of diabetes mellitus, ranging from human genetic studies through clinical physiology and trials to diabetes epidemiology. We do not publish original animal or cell culture studies unless they are part of a study of clinical diabetes involving humans. Categories of publication include research articles, reviews, editorials, commentaries, and correspondence. All material is peer-reviewed.
We aim to disseminate knowledge about diabetes research with the goal of improving the management of people with diabetes. The journal therefore seeks to provide a forum for the exchange of ideas between clinicians and researchers worldwide. Topics covered are of importance to all healthcare professionals working with people with diabetes, whether in primary care or specialist services.
Surplus generated from the sale of Diabetic Medicine is used by Diabetes UK to know diabetes better and fight diabetes more effectively on behalf of all people affected by and at risk of diabetes as well as their families and carers.”
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