What is missing in a manuscript?

As academic publication becomes increasingly more competitive for both authors and also the publishers, quality of a manuscript is the most critical element for acceptance through the intensive reviewing process. Manuscripts are increasing in number, but fewer are distinguished from the enormous large pool of them. It is logical for authors to ask what is needed or missing in a manuscript for a good quality to be judged by professional peers? Very briefly, the reproducibility of the results is the basic element and foundation stone for science, and, more importantly, the soul of a manuscript, more than often, is missing. Because of this, I would like to discuss contents of a manuscript in this communication as my focal point in a hope to assist authors to improve manuscript quality and submission success rate.