高脂饮食持续时间对大鼠附睾脂肪细胞增生的影响。

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Obesity research & clinical practice Pub Date : 2025-01-01 DOI:10.1016/j.orcp.2025.02.001

Zhaoxin Wang , Xiujuan Liu , Lei Sheng , Yuting Xie , Wanyu Feng , Li Yu

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引用次数: 0

摘要

背景：高脂肪饮食（HFD）有助于肥胖，并提高成熟脂肪细胞标志物的表达。然而，HFD对脂肪细胞增生的影响仍存在争议。这可能是由于HFD持续时间的变化。本研究旨在探讨不同时间HFD对肥胖大鼠脂肪细胞增生及成熟脂肪细胞相关标志物表达的影响。方法：将32只sd大鼠分为4组：B组（标准饮食对照组）、H1组（HFD组）、H2组（HFD组）、H3组（HFD组）。我们评估了附睾脂肪细胞的形态学变化，使用酶联免疫吸附测定（ELISA）试剂盒测量血清炎症标志物，并使用western blotting定量脂肪细胞增生和成熟标志物。结果：我们观察到体重、附睾脂肪重量、血清瘦素、TNF-α、IL-6、鸢尾素、PPARγ、脂联素和FNDC5蛋白表达在HFD治疗8周后渐进式增加。12周的HFD干预导致鸢尾素、PPARγ、脂联素和FNDC5显著降低。同时，perilipin A和ATGL的表达随HFD的延长而降低。结论：我们的研究结果表明，HFD持续时间显著影响肥胖大鼠附睾脂肪细胞的增生能力。具体来说，4周的高脂饮食没有改变脂肪细胞增生的能力，而8周的饮食则增强了这种能力。有趣的是，较长的饮食持续时间（12周）导致脂肪细胞增生减少。

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Effects of duration of high-fat diet on adipocyte hyperplasia in rat epididymis

Background

High-fat diet (HFD) contributes to obesity and enhances the expression of mature adipocyte markers. However, the effect of HFD on adipocyte hyperplasia remains controversial. This may be due to variations in the duration of HFD. This study aimed to investigate the effects of different durations of HFD on adipocyte hyperplasia and the expression of mature adipocyte-related markers in obese rats.

Methods

We divided 32 Sprague-Dawley rats into four groups: B (standard diet control), H1 (HFD for four weeks), H2 (HFD for eight weeks), and H3 (HFD for 12 weeks). We evaluated the morphological changes in epididymal fat cells, measured serum inflammatory markers using enzyme-linked immunosorbent assay (ELISA) kits, and quantified adipocyte hyperplasia and maturation markers using western blotting.

Results

We observed progressive increases in body weight, epididymal fat weight, serum leptin, TNF-α, IL-6, irisin, PPARγ, adiponectin, and FNDC5 protein expression over 8 weeks of HFD. 12 weeks of HFD intervention resulted in significant decreases in irisin, PPARγ, adiponectin, and FNDC5. Concurrently, the expression of perilipin A and ATGL declined with prolonged HFD.

Conclusions

Our results suggest that the duration of HFD significantly affects adipocyte ability to undergo hyperplasia in the epididymis of obese rats. Specifically, 4 weeks of HFD did not change the capacity for adipocyte hyperplasia, while 8 weeks of the diet enhanced this capacity. Interestingly, a longer diet duration (12 weeks) led to a decrease in adipocyte hyperplasia.

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来源期刊

Obesity research & clinical practice 医学-内分泌学与代谢

CiteScore

7.10

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： The aim of Obesity Research & Clinical Practice (ORCP) is to publish high quality clinical and basic research relating to the epidemiology, mechanism, complications and treatment of obesity and the complication of obesity. Studies relating to the Asia Oceania region are particularly welcome, given the increasing burden of obesity in Asia Pacific, compounded by specific regional population-based and genetic issues, and the devastating personal and economic consequences. The journal aims to expose health care practitioners, clinical researchers, basic scientists, epidemiologists, and public health officials in the region to all areas of obesity research and practice. In addition to original research the ORCP publishes reviews, patient reports, short communications, and letters to the editor (including comments on published papers). The proceedings and abstracts of the Annual Meeting of the Asia Oceania Association for the Study of Obesity is published as a supplement each year.