脑及外周组织中adropin水平随衰老的变化。

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-02-06 DOI:10.1016/j.neulet.2025.138150

Changjun Yang, John Aaron Howell, Lei Liu, Rachel E. Gunraj, Eduardo Candelario-Jalil

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引用次数: 0

摘要

Adropin是一种生物活性肽，存在于大脑和各种周围组织中。有证据表明，衰老会显著降低大脑adropin水平，提高adropin水平的干预措施可能有助于缓解与年龄相关的神经系统疾病，如缺血性中风和认知能力下降。然而，衰老对外周组织adropin水平的影响及其与大脑神经识别分子NB-3/contactin-6的关系尚不清楚。在这项研究中，我们使用免疫印迹法对幼龄雄性小鼠（8-10 周）和老年雄性小鼠（18-20 个月）的大脑和外周组织（肝、肺、肾、脾、回肠、结肠）中的adropin进行量化。结果表明，老龄小鼠的脑促肾上腺素水平显著下降，而与年轻对照组相比，外周组织没有明显变化。此外，NB-3/contactin-6（一种潜在的促肾上腺素受体和Notch1配体）的水平在老年大脑中较低。共免疫沉淀表明adropin与脑NB-3存在物理关联。值得注意的是，与年龄相关的脑促激素减少与氧化应激标志物（gp91phox和4-羟基壬烯醛）的增加有关。我们提供了第一个证据，表明衰老与大脑中而不是外周组织中adropin和NB-3的同时丢失有关。维持大脑促肾上腺素水平的干预措施有助于减缓大脑的衰老过程，减轻与年龄相关的神经功能障碍。

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Changes in adropin levels in brain and peripheral tissues with aging

Adropin is a bioactive peptide found in the brain and various peripheral tissues. Evidence suggests that aging significantly decreases brain adropin levels, and interventions that elevate adropin may help alleviate age-related neurological disorders such as ischemic stroke and cognitive decline. However, the impact of aging on peripheral tissue adropin levels and its relationship with the neural recognition molecule NB-3/contactin-6 in the brain remains unclear. In this study, we quantified adropin using immunoblotting in brain and peripheral tissues (liver, lung, kidney, spleen, ileum, colon) from young (8–10 weeks) and aged (18–20 months) male mice. Results indicated a significant decrease in brain adropin levels in aged mice, while peripheral tissues showed no significant changes compared to young controls. Additionally, levels of NB-3/contactin-6, a potential adropin receptor and Notch1 ligand, were lower in aged brains. Co-immunoprecipitation demonstrated that adropin physically associates with brain NB-3. Notably, the age-related reduction in brain adropin correlates with increased oxidative stress markers (gp91^phox and 4-hydroxynonenal). We provide the first evidence that aging is linked to a concurrent loss of adropin and NB-3 in the brain but not in peripheral tissues. Interventions to maintain brain adropin levels could help mitigate the brain’s aging process and alleviate age-related neurological dysfunction.

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

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