Histone acetyltransferase Sas3 in Phomopsis liquidambaris promotes spermidine biosynthesis against Fusarium graminearum in wheat.

Key message: Spermidine production in the endophytic fungus Phomopsis liquidambaris is regulated by Sas3, and spermidine promotes resistance to Fusarium graminearum by increasing the expression of immune-related indicators in wheat. Fusarium head blight (FHB) is a common wheat disease caused mainly by Fusarium graminearum. The present study showed that overexpression of the histone acetyltransferase Sas3 in Phomopsis liquidambaris regulated the synthesis of spermidine and promoted resistance to F. graminearum in wheat. Sas3 localized in the nucleus plays a key role in acetylating lysines 9 and 14 of histone H3 (H3K9 and H3K14) and clearly promotes the development and growth of P. liquidambaris in the overexpression strain OE-Sas3 and knockout strain Ko-Sas3. The OE-Sas3 strain promoted the growth of wheat seedlings and increased the level of reactive oxygen species (ROS) pumps, which increased the activities of the catalase (CAT) and peroxidase (POD) and the expression levels of genes involved in the jasmonic acid, ethylene, and salicylic acid pathways. Furthermore, OE-Sas3 increased the level of resistance of wheat to F. graminearum through the positive regulation of spermidine biosynthesis, which reduced the incidence of wheat spike disease from 76 to 54% and that of grain disease from 52.35 to 32.68%. This study provides a new perspective for the application of P. liquidambaris as a biocontrol agent via rational design and improved FHB resistance.