模拟微重力条件下花斑茅多糖肽对4T1乳腺肿瘤生长和转移的抑制作用

IF 1.3 4区 工程技术 Q2 ENGINEERING, AEROSPACE
Morteza Alemi, Firooz Samadi, Alireza Madjid Ansari, Zahra Hajebrahimi
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引用次数: 0

摘要

在妇女中流行的一种癌症是乳腺癌,造成了大量与癌症有关的死亡。花椰菜提取物具有显著的抗癌作用。此外,微重力可以影响癌细胞行为的各个方面,包括生长、迁移、增殖、存活和凋亡。本研究旨在评估CV多糖肽联合阿霉素在模拟微重力(S-µg)和无S-µg条件下对4T1癌细胞的作用。将80只雌性近交系BALB/c小鼠分为8个实验组,分别为对照组、CV多糖肽(T, 1 g/kg BW)、阿霉素注射液(Dox, 0.5 mg/kg BW)、Dox + T、S-µg (Mic)、Mic + D、Mic + T和Mic + D + T。随后,使用Western blot、TUNEL试验、免疫组织化学和H&;E染色技术表达肿瘤生物标志物和细胞死亡。与对照组相比,暴露于S-µg可降低肿瘤生长,但其作用小于受CV和Dox多糖肽影响的组。Dox和CV联合用药可显著抑制4T1乳腺肿瘤的生长(p < 0.001)。与对照组相比,Dox + T组肿瘤体积减小(p < 0.001)。同时能有效抑制肿瘤生长,抑制Ki-67、VEGF、MMP-2、MMP-9的表达。Dox + T可延长荷瘤小鼠的平均生存时间。Dox + T显著增加了caspase-3、caspase-8和caspase-9的裂解,证实了细胞凋亡的发生(p < 0.001)。综上所述,Dox + T可以作为一种安全的抗癌补充剂,直接导致癌细胞凋亡。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Inhibitory Potential Effects of Polysaccharide Peptide from Coriolus versicolor on the Growth and Metastasis of 4T1 Breast Tumors Under Simulated Microgravity Conditions

The form of cancer that is prevalent among women is breast cancer and is responsible for a significant number of cancer-related deaths. Coriolus versicolor (CV) extract has demonstrated notable anti-cancer properties. Additionally, microgravity can influence various aspects of cancer cell behavior, including growth, migration, proliferation, survival, and apoptosis. This research aimed to assess the efficacy of polysaccharide peptide from CV in combination with doxorubicin under simulated microgravity (S-µg) conditions and without S-µg on 4T1 cancer cells. A number of 80 female inbred BALB/c mice were divided into 8 experiment groups, including Control, polysaccharide peptide from CV (T, 1 g/kg BW), doxorubicin injection (Dox, 0.5 mg/kg BW), Dox + T, S-µg (Mic), Mic + D, Mic + T, and Mic + D + T. Subsequently, tumor biomarkers and cell death were expressed using Western blot, TUNEL assay, immunohistochemistry, and H&E staining techniques. Exposure to S-µg reduced tumor growth compared to the control group, but its effect was less than that of the groups affected by polysaccharide peptide from CV and Dox. The Dox and CV combination could significantly (p < 0.001) inhibit 4T1 breast tumors’ growth. The Dox + T group exhibited a decrease in the tumors’ volume compared to control (p < 0.001). Meanwhile, it could effectively suppress tumor growth and Ki-67, VEGF, MMP-2, and MMP-9 expression. Also, Dox + T could increase the mean survival time of the tumor-bearing mice. Dox + T demonstrated a meaningful increase in cleavage of caspase-3, caspase-8, and caspase-9, confirming the occurrence of apoptosis (p < 0.001). Taking together, Dox + T can be a safe anti-cancer supplement that can cause direct apoptosis to the cancer cells.

Graphical Abstract

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来源期刊
Microgravity Science and Technology
Microgravity Science and Technology 工程技术-工程:宇航
CiteScore
3.50
自引率
44.40%
发文量
96
期刊介绍: Microgravity Science and Technology – An International Journal for Microgravity and Space Exploration Related Research is a is a peer-reviewed scientific journal concerned with all topics, experimental as well as theoretical, related to research carried out under conditions of altered gravity. Microgravity Science and Technology publishes papers dealing with studies performed on and prepared for platforms that provide real microgravity conditions (such as drop towers, parabolic flights, sounding rockets, reentry capsules and orbiting platforms), and on ground-based facilities aiming to simulate microgravity conditions on earth (such as levitrons, clinostats, random positioning machines, bed rest facilities, and micro-scale or neutral buoyancy facilities) or providing artificial gravity conditions (such as centrifuges). Data from preparatory tests, hardware and instrumentation developments, lessons learnt as well as theoretical gravity-related considerations are welcome. Included science disciplines with gravity-related topics are: − materials science − fluid mechanics − process engineering − physics − chemistry − heat and mass transfer − gravitational biology − radiation biology − exobiology and astrobiology − human physiology
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