在炎症基础的生殖病理中，f2 -异前列腺素是否比丙二醛更好地标记精液脂质过氧化？

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2025-02-08 DOI:10.1016/j.cyto.2025.156889

Elena Moretti , Cinzia Signorini , Silvia Menchiari , Laura Liguori , Roberta Corsaro , Laura Gambera , Giulia Collodel

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引用次数: 0

摘要

许多男性生殖疾病和部分未确诊的不育症都有氧化应激（OS）的病因，具有高活性氧和细胞因子浓度。缺乏可靠的生物标志物来量化氧化损伤是男性不育领域的一个关键问题。在这项观察性研究中，对46例精索静脉曲张、泌尿生殖系统感染、特发性不育症患者和11名育龄男性的精浆中IL-1β和OS标志物丙二醛（MDA）和f2 -异前列腺素（F2-IsoPs）进行了定量分析。按照WHO指南进行精液分析，ELISA法测定IL-1β， HPLC法测定MDA， GC/NICI-MS法测定F2-IsoPs。F2-IsoPs在可育和不育受试者的精子中免疫定位。结果显示F2-IsoP、MDA和IL-1β精液水平呈两两正相关(p <；0.001)，并与精子参数呈负相关(p <；0.001)。然后，根据不孕原因对研究人群进行分组，并比较不同组和对照样本之间的变量。精索静脉曲张患者精液IL-1β、F2-IsoPs和MDA显著升高(p <；0.001, MDA p <；0.01)和泌尿生殖系统感染(p <；0.001, IL-1β p <；0.01)。F2-IsoPs似乎比MDA更准确地区分不同的条件，特别是特发性不孕。ROC曲线显示，所分析的三个指标能够区分可育和不育患者。免疫荧光研究显示，可育男性精子中F2-IsoPs的含量较低，在不育患者精子的尾部和细胞质残留物中有明显的标记。综上所述，该数据证实F2-IsoP水平是精浆中OS的合适标记物，甚至比MDA更准确，可用于临床环境中OS的测量。

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Are F2-isoprostanes a better marker of semen lipid peroxidation than MDA in reproductive pathologies with inflammatory basis?

Many male reproductive pathologies and a part of undiagnosed infertility share an oxidative stress (OS) etiology with high reactive oxygen species and cytokine concentrations. The lack of reliable biomarkers to quantify oxidative injury is a crucial problem in the field of male infertility. In this observational study, IL-1β and the OS markers malondialdehyde (MDA) and F₂-isoprostanes (F₂-IsoPs) were quantified in seminal plasma of 46 infertile patients with varicocele, genitourinary infections, idiopathic infertility, and 11 fertile men. Semen analysis was performed following WHO guidelines, IL-1β was determined by ELISA, MDA was quantified by HPLC, and F₂-IsoPs by GC/NICI-MS analysis. F₂-IsoPs were immunolocalized in spermatozoa of fertile and infertile subjects. Results indicated that F₂-IsoP, MDA, and IL-1β seminal levels positively correlated pairwise (p < 0.001) and showed negative correlations with sperm parameters (p < 0.001). Then, the studied population was grouped following the cause of infertility and the variables were compared between the different groups and a control sample. Seminal IL-1β, F₂-IsoPs, and MDA were significantly higher in varicocele (p < 0.001, for MDA p < 0.01) and genitourinary infections (p < 0.001, for IL-1β p < 0.01) groups than those observed in fertile subjects. F₂-IsoPs seemed to discriminate more accurately than MDA the different conditions, in particular idiopathic infertility. ROC curves demonstrated that the three analyzed indices were able to discriminate fertile and infertile patients. The immunofluorescence studies showed a low presence of F₂-IsoPs in spermatozoa of fertile men and an evident labeling in the tail, and cytoplasmic residues of spermatozoa from infertile patients. In conclusion, this data confirmed that F₂-IsoP level is a suitable marker of OS in seminal plasma, even more accurate than MDA and can be proposed for measuring OS in the clinical setting.

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.