合成程序抗氧化输送由驯化的皮肤共生。

Cell systems Pub Date : 2025-02-19 Epub Date: 2025-02-06 DOI:10.1016/j.cels.2025.101169
Guillermo Nevot, Javier Santos-Moreno, Nil Campamà-Sanz, Lorena Toloza, Cristóbal Parra-Cid, Patrick A M Jansen, Içvara Barbier, Rodrigo Ledesma-Amaro, Ellen H van den Bogaard, Marc Güell
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引用次数: 0

摘要

细菌是一种很有前途的治疗疾病的动态传递系统。在皮肤中,痤疮角质杆菌在毛囊内的相关位置使这种细菌成为皮肤生物技术应用的一个有吸引力的基础。在这里,我们为这种传统上难以处理的细菌的工程提供了一个遗传工具箱,包括基本的基因表达工具,生物控制策略,无标记基因工程和动态转录调控。作为概念的证明,我们开发了一种抗氧化剂分泌菌株,能够减少紫外线应激模型中的氧化应激。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synthetically programmed antioxidant delivery by a domesticated skin commensal.

Bacteria represent a promising dynamic delivery system for the treatment of disease. In the skin, the relevant location of Cutibacterium acnes within the hair follicle makes this bacterium an attractive chassis for dermal biotechnological applications. Here, we provide a genetic toolbox for the engineering of this traditionally intractable bacterium, including basic gene expression tools, biocontainment strategies, markerless genetic engineering, and dynamic transcriptional regulation. As a proof of concept, we develop an antioxidant-secreting strain capable of reducing oxidative stress in a UV stress model.

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