低强度脉冲超声刺激肠道改善2型糖尿病胰岛素抵抗。

IF 2.4 3区医学 Q2 ACOUSTICS

Ultrasound in Medicine and Biology Pub Date : 2025-02-06 DOI:10.1016/j.ultrasmedbio.2025.01.004

Huan Wang , Min He , Badamgarav Bat-Erdene, Ying Li, Dean Ta

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引用次数: 0

摘要

目的：超声刺激内脏和周围神经在调节血糖代谢方面显示出良好的潜力。本研究旨在评估低强度脉冲超声刺激（LIPUS）对肠道改善2型糖尿病（T2DM）胰岛素抵抗的有效性。方法：将正常和T2DM小鼠C57BL/6J随机分为对照组、T2DM -sham组和T2DM - lipus组。T2D-LIPUS组给予肠内LIPUS刺激。参数为：1 MHz频率，1.0 kHz脉冲重复频率（PRF）， 20%占空比，100 mW/cm²强度空间平均时间平均（ISATA），每次20分钟，每周5天，持续4周。结果：血糖分析显示，T2D-LIPUS组小鼠的葡萄糖耐量试验（GTT）和胰岛素耐量试验（ITT）曲线下面积（AUC）（p < 0.001）、HOMA-IR （p < 0.001）和空腹血清胰岛素水平（p < 0.01）均明显低于T2D-sham组。LIPUS有效降低T2DM小鼠血清中IL-1β (p < 0.001)、TNF-α (p < 0.01)水平及肠道中IL-1β (p < 0.01)、IL-18 (p < 0.001) mRNA表达水平。此外，Western blot分析显示，LIPUS处理小鼠肠道组织中NLRP3、caspase-1和GSDMD-N蛋白水平降低。结论：LIPUS可通过抑制NLRP3/Caspase-1/GSDMD信号通路，减轻炎症和细胞凋亡，改善胰岛素抵抗。本研究介绍了一种新的非药物治疗T2DM的方法。

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Low-intensity Pulsed Ultrasound Stimulation of the Intestine Improves Insulin Resistance in Type 2 Diabetes

Objective

Ultrasound stimulation of internal organs and peripheral nerves has demonstrated promising potential in regulating blood glucose metabolism. This study aims to assess the effectiveness of low-intensity pulsed ultrasound stimulation (LIPUS) on intestine in improving insulin resistance with type 2 diabetes mellitus (T2DM).

Methods

C57BL/6J mice, both normal and T2DM, were randomly divided into three groups: Control, T2D-sham, and T2D-LIPUS. The T2D-LIPUS group received LIPUS stimulation in the intestine. The parameters were as follows: 1 MHz frequency, 1.0 kHz pulse repetition frequency (PRF), 20% duty cycle, 100 mW/cm² intensity spatial average temporal average (I_SATA), for 20 minutes per session, five days per week, over four weeks.

Results

Blood glucose analysis indicated that mice in the T2D-LIPUS group displayed significantly lower area under the curve (AUC) of glucose tolerance tests (GTT) and insulin tolerance tests (ITT) (p < 0.001), HOMA-IR (p < 0.001), and fasting serum insulin levels (p < 0.01) compared to the T2D-sham group. LIPUS treatment effectively lowered serum levels of IL-1β (p < 0.001) and TNF-α (p < 0.01) along with mRNA expression levels of IL-1β (p < 0.01) and IL-18 (p < 0.001) in the intestines of T2DM mice. Additionally, Western blot analysis revealed a reduction in the protein levels of NLRP3, caspase-1, and GSDMD-N in the intestinal tissues of mice treated with LIPUS.

Conclusion

These findings suggest that LIPUS can reduce inflammation and cellular apoptosis, while improving insulin resistance by inhibiting the NLRP3/Caspase-1/GSDMD signaling pathway. This research introduces a novel, non-pharmacological approach for managing T2DM.

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来源期刊

Ultrasound in Medicine and Biology 医学-核医学

CiteScore

6.20

自引率

6.90%

发文量

325

审稿时长

70 days

期刊介绍： Ultrasound in Medicine and Biology is the official journal of the World Federation for Ultrasound in Medicine and Biology. The journal publishes original contributions that demonstrate a novel application of an existing ultrasound technology in clinical diagnostic, interventional and therapeutic applications, new and improved clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and the interactions between ultrasound and biological systems, including bioeffects. Papers that simply utilize standard diagnostic ultrasound as a measuring tool will be considered out of scope. Extended critical reviews of subjects of contemporary interest in the field are also published, in addition to occasional editorial articles, clinical and technical notes, book reviews, letters to the editor and a calendar of forthcoming meetings. It is the aim of the journal fully to meet the information and publication requirements of the clinicians, scientists, engineers and other professionals who constitute the biomedical ultrasonic community.