角膜上皮离体模型真菌感染监测及聚醚砜膜采集检测白色念珠菌和烟曲霉。

IF 5.5 3区 医学 Q1 IMMUNOLOGY
Sarp Orgul, Angela Gómez Bedoya, Víctor Felipe Pérez, Daniella R Mora, Alfonso L Sabater, Darlene Miller, Miguel Holgado
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引用次数: 0

摘要

在本文中,我们报告了人类离体模型烟曲霉和白色念珠菌感染的角膜。我们展示了通过光学和免疫荧光显微镜监测角膜真菌生长的能力。此外,我们建立了一种从真菌接种的角膜表面收集真菌抗原的聚醚砜(PES)膜样品收集器的方法。免疫荧光显微镜直接在角膜组织和PES膜样品收集器上进行。对于后一种情况,我们设计了一种独创的特别方法,将用于收集的膜附着在标准玻璃载玻片上,我们称之为玻璃载玻片KIT。这使得简单的免疫分析方案易于处理并提高了特异性识别相应真菌感染的效率。因此,我们首先观察到真菌在角膜组织中的监测能力,其次证明真菌抗原在PES膜上的吸附。因此,我们首次报道了在易于使用的PES膜基玻片试剂盒中通过简单的免疫荧光特异性检测真菌性角膜感染,作为微生物培养的替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fungal infection monitoring on corneal epithelium ex vivo model and its collection over polyethersulfone membrane for detecting Candida albicans and Aspergillus fumigatus.

In this paper we report a human ex vivo model of Aspergillus fumigatus and Candida albicans infection of the cornea. We demonstrate the ability to monitor fungal growth on the cornea through optical and immunofluorescence microscopy. Additionally, we establish a method for collection of fungal antigens in polyethersulfone (PES) membrane-based sample collectors from the surface of fungal inoculated corneas. Immunofluorescence microscopy was performed directly on both corneal tissue and PES membrane sample collectors. For the latter case, we devised an original ad-hoc method by attaching the membranes used for the collection on standard glass slides, which we call glass slide KIT. This enabled easy handling and improved efficiency for specifically recognizing the corresponding fungal infections by simple immunoassay protocols. As a result, we firstly observe the ability to monitor fungi in corneal tissue, and secondly demonstrate the adsorption of fungal antigens onto PES membranes. Thus, we report for the first time the specific detection of fungal corneal infections in easy to use PES membrane based glass slide KITs through simple immunofluorescence, as an alternative to microbiological culture.

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来源期刊
CiteScore
10.60
自引率
0.00%
发文量
29
审稿时长
1 months
期刊介绍: Medical Microbiology and Immunology (MMIM) publishes key findings on all aspects of the interrelationship between infectious agents and the immune system of their hosts. The journal´s main focus is original research work on intrinsic, innate or adaptive immune responses to viral, bacterial, fungal and parasitic (protozoan and helminthic) infections and on the virulence of the respective infectious pathogens. MMIM covers basic, translational as well as clinical research in infectious diseases and infectious disease immunology. Basic research using cell cultures, organoid, and animal models are welcome, provided that the models have a clinical correlate and address a relevant medical question. The journal also considers manuscripts on the epidemiology of infectious diseases, including the emergence and epidemic spreading of pathogens and the development of resistance to anti-infective therapies, and on novel vaccines and other innovative measurements of prevention. The following categories of manuscripts will not be considered for publication in MMIM: submissions of preliminary work, of merely descriptive data sets without investigation of mechanisms or of limited global interest, manuscripts on existing or novel anti-infective compounds, which focus on pharmaceutical or pharmacological aspects of the drugs, manuscripts on existing or modified vaccines, unless they report on experimental or clinical efficacy studies or provide new immunological information on their mode of action, manuscripts on the diagnostics of infectious diseases, unless they offer a novel concept to solve a pending diagnostic problem, case reports or case series, unless they are embedded in a study that focuses on the anti-infectious immune response and/or on the virulence of a pathogen.
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