Leema Rose Mary Devasahayam, Marie Arockianathan Pushpam, Lawrance Antonysamy, Senthamizhselvan Anbazhagan
{"title":"木脚草粘液的分离、鉴定与评价。叶子。","authors":"Leema Rose Mary Devasahayam, Marie Arockianathan Pushpam, Lawrance Antonysamy, Senthamizhselvan Anbazhagan","doi":"10.2174/0113862073345969241129043350","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Many plant-derived compounds have been used as pharmaceutical excipients due to their various functional properties. One such plant product is mucilage, which has created great interest among researchers to be explored for numerous biological applications.</p><p><strong>Objectives: </strong>The objective of the present study was to isolate mucilage from the leaves of Pedalium murex Linn. (family; Pedaliaceae). The obtained mucilage was characterized physiochemically and evaluated for its antioxidant and anticancer properties.</p><p><strong>Methods: </strong>The obtained mucilage was screened for its phytochemicals and characterized physiochemically using FTIR, XRD, DSC, and SEM. Its antioxidant property was analyzed using DPPH and FRAP assays. The anticancer activity of the mucilage was evaluated against colon and cervical cancer cell lines using an MTT assay.</p><p><strong>Results: </strong>The phytochemical screening of the mucilage demonstrated the ability to reduce sugars, flavonoids, amino acids, tannins, saponins, and steroids. The yield percentage of mucilage was 24%, and it has a swelling index of 10. It was found to be soluble in warm water and showed a pH of 9. The FTIR spectra of mucilage showed characteristic peaks of its functional groups. The SEM image showed a porous and rough morphological surface of mucilage. The obtained mucilage demonstrated antioxidant activity by DPPH and FRAP assays and exhibited anticancer activity against cervical and colon cancer cell lines using the MTT assay.</p><p><strong>Conclusion: </strong>Mucilage contains many phytochemicals with various functional properties like antioxidant and anti-cancer activities. Thus, the isolated mucilage from Pedalium murex Linn. showed promising characteristics that could be tailor-made for various biological applications.</p>","PeriodicalId":10491,"journal":{"name":"Combinatorial chemistry & high throughput screening","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation, Characterization, and Evaluation of the Mucilage from Pedalium murex Linn. Leaves.\",\"authors\":\"Leema Rose Mary Devasahayam, Marie Arockianathan Pushpam, Lawrance Antonysamy, Senthamizhselvan Anbazhagan\",\"doi\":\"10.2174/0113862073345969241129043350\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Many plant-derived compounds have been used as pharmaceutical excipients due to their various functional properties. One such plant product is mucilage, which has created great interest among researchers to be explored for numerous biological applications.</p><p><strong>Objectives: </strong>The objective of the present study was to isolate mucilage from the leaves of Pedalium murex Linn. (family; Pedaliaceae). The obtained mucilage was characterized physiochemically and evaluated for its antioxidant and anticancer properties.</p><p><strong>Methods: </strong>The obtained mucilage was screened for its phytochemicals and characterized physiochemically using FTIR, XRD, DSC, and SEM. Its antioxidant property was analyzed using DPPH and FRAP assays. The anticancer activity of the mucilage was evaluated against colon and cervical cancer cell lines using an MTT assay.</p><p><strong>Results: </strong>The phytochemical screening of the mucilage demonstrated the ability to reduce sugars, flavonoids, amino acids, tannins, saponins, and steroids. The yield percentage of mucilage was 24%, and it has a swelling index of 10. It was found to be soluble in warm water and showed a pH of 9. The FTIR spectra of mucilage showed characteristic peaks of its functional groups. The SEM image showed a porous and rough morphological surface of mucilage. The obtained mucilage demonstrated antioxidant activity by DPPH and FRAP assays and exhibited anticancer activity against cervical and colon cancer cell lines using the MTT assay.</p><p><strong>Conclusion: </strong>Mucilage contains many phytochemicals with various functional properties like antioxidant and anti-cancer activities. 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Isolation, Characterization, and Evaluation of the Mucilage from Pedalium murex Linn. Leaves.
Background: Many plant-derived compounds have been used as pharmaceutical excipients due to their various functional properties. One such plant product is mucilage, which has created great interest among researchers to be explored for numerous biological applications.
Objectives: The objective of the present study was to isolate mucilage from the leaves of Pedalium murex Linn. (family; Pedaliaceae). The obtained mucilage was characterized physiochemically and evaluated for its antioxidant and anticancer properties.
Methods: The obtained mucilage was screened for its phytochemicals and characterized physiochemically using FTIR, XRD, DSC, and SEM. Its antioxidant property was analyzed using DPPH and FRAP assays. The anticancer activity of the mucilage was evaluated against colon and cervical cancer cell lines using an MTT assay.
Results: The phytochemical screening of the mucilage demonstrated the ability to reduce sugars, flavonoids, amino acids, tannins, saponins, and steroids. The yield percentage of mucilage was 24%, and it has a swelling index of 10. It was found to be soluble in warm water and showed a pH of 9. The FTIR spectra of mucilage showed characteristic peaks of its functional groups. The SEM image showed a porous and rough morphological surface of mucilage. The obtained mucilage demonstrated antioxidant activity by DPPH and FRAP assays and exhibited anticancer activity against cervical and colon cancer cell lines using the MTT assay.
Conclusion: Mucilage contains many phytochemicals with various functional properties like antioxidant and anti-cancer activities. Thus, the isolated mucilage from Pedalium murex Linn. showed promising characteristics that could be tailor-made for various biological applications.
期刊介绍：
Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal:
Target identification and validation
Assay design, development, miniaturization and comparison
High throughput/high content/in silico screening and associated technologies
Label-free detection technologies and applications
Stem cell technologies
Biomarkers
ADMET/PK/PD methodologies and screening
Probe discovery and development, hit to lead optimization
Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries)
Chemical library design and chemical diversity
Chemo/bio-informatics, data mining
Compound management
Pharmacognosy
Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products)
Natural Product Analytical Studies
Bipharmaceutical studies of Natural products
Drug repurposing
Data management and statistical analysis
Laboratory automation, robotics, microfluidics, signal detection technologies
Current & Future Institutional Research Profile
Technology transfer, legal and licensing issues
Patents.
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