基于化学生物学方法的中药物质基础研究进展

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-02-07 DOI:10.1002/bmc.70004

Xinyue Bai, Huimin Lin, Ziqi Yang, Fan He, Jianru Chen, Hua Zhou, Yufeng Huang

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引用次数: 0

摘要

化学生物学方法是利用化学研究方法和思想，在分子水平上研究生命和生物医学的尖端技术。近年来，它已逐渐应用于中药化学标记物的筛选。本文综述了常用的化学生物学方法，包括细胞膜色谱法、亲和超滤法、磁珠富集法、毛细管电泳法、分子排斥色谱法、固定化融合靶亲和色谱法等在中药研究中的原理及应用。应用化学生物学方法筛选中药化学标志物，具有操作简单快速、结果特异性强、重现性好、灵敏度高等优点，可实现高通量筛选。

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Research Progress on the Material Basis of Traditional Chinese Medicines Based on Chemical Biology Methods

The chemical biology method is a cutting-edge technology that utilizes chemical research methods and ideas to study life and biomedicine at the molecular level. Recently, it has gradually been applied to screening chemical markers in traditional Chinese medicines. This article reviews the principles and applications of common chemical biology methods, including cell membrane chromatography, affinity ultrafiltration, magnetic bead enrichment, capillary electrophoresis, molecular exclusion chromatography, immobilized fusion target affinity chromatography, etc., in traditional Chinese medicines research. Applying chemical biology methods to screen chemical markers in traditional Chinese medicines has the advantages of simple and fast operation, strong specificity of results, good reproducibility, and high sensitivity, which can achieve high-throughput screening.

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.