{"title":"A型肉毒毒素对卵巢扭转潜在治疗作用的研究：来自大鼠模型的发现","authors":"Çağlayan Ateş, Berna Dilbaz, Seval Yılmaz Ergani, Pelin Seçken, Caner Köse","doi":"10.1111/aji.70057","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>The aim of this study was to investigate the effects of botulinum toxin A on histological abnormalities, loss of ovarian reserve, and angiogenesis within the ovarian torsion–detorsion paradigm.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>A total of 45 female Wistar albino rats were used for the study. The rats were randomly divided into five groups (<i>n</i> = 9 in each group): Control, Torsion, Torsion + Detorsion, Torsion + Detorsion + Botulinum toxin, and Botulinum toxin only. Follicular cell degeneration, vascular congestion, hemorrhage, inflammation, and vascular endothelial growth factor (VEGF) expression scores were compared histopathologically between the groups. Follicle numbers were compared. Anti-mullerian hormone levels were measured before and after torsion, detorsion, and treatments.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Follicular cell degeneration, vascular congestion, hemorrhage, inflammation, and VEGF expression differed significantly between groups for each score (<i>p </i>< 0.001). The VEGF expression score was found to increase more in the torsion + detorsion + botulinum toxin group than in the torsion + detorsion group (2.67 ± 0.71 and 2.22 ± 0.67, respectively). There was a significant difference between the groups in secondary follicle counts (<i>p</i> = 0.04). The groups showed statistically significant differences in their anti-mullerian hormone levels (<i>p </i>< 0.001).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>This initial investigation of the effects of botulinum toxin on ovarian torsion (OT) has shown that botulinum toxin type A has the potential to be a useful adjunct treatment for fertility preservation. Our results proved the ovarian protective effect of botulinum toxin type A in rats with OT. Therefore, botulinum toxin type A may be a potential preventive agent against ovarian damage caused by torsion in the future.</p>\n </section>\n </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"93 2","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of the Potential Treatment Effect of Botulinum Toxin Type A in Ovarian Torsion: Findings From the Rat Model\",\"authors\":\"Çağlayan Ateş, Berna Dilbaz, Seval Yılmaz Ergani, Pelin Seçken, Caner Köse\",\"doi\":\"10.1111/aji.70057\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>The aim of this study was to investigate the effects of botulinum toxin A on histological abnormalities, loss of ovarian reserve, and angiogenesis within the ovarian torsion–detorsion paradigm.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>A total of 45 female Wistar albino rats were used for the study. The rats were randomly divided into five groups (<i>n</i> = 9 in each group): Control, Torsion, Torsion + Detorsion, Torsion + Detorsion + Botulinum toxin, and Botulinum toxin only. Follicular cell degeneration, vascular congestion, hemorrhage, inflammation, and vascular endothelial growth factor (VEGF) expression scores were compared histopathologically between the groups. Follicle numbers were compared. Anti-mullerian hormone levels were measured before and after torsion, detorsion, and treatments.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Follicular cell degeneration, vascular congestion, hemorrhage, inflammation, and VEGF expression differed significantly between groups for each score (<i>p </i>< 0.001). The VEGF expression score was found to increase more in the torsion + detorsion + botulinum toxin group than in the torsion + detorsion group (2.67 ± 0.71 and 2.22 ± 0.67, respectively). There was a significant difference between the groups in secondary follicle counts (<i>p</i> = 0.04). The groups showed statistically significant differences in their anti-mullerian hormone levels (<i>p </i>< 0.001).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>This initial investigation of the effects of botulinum toxin on ovarian torsion (OT) has shown that botulinum toxin type A has the potential to be a useful adjunct treatment for fertility preservation. Our results proved the ovarian protective effect of botulinum toxin type A in rats with OT. Therefore, botulinum toxin type A may be a potential preventive agent against ovarian damage caused by torsion in the future.</p>\\n </section>\\n </div>\",\"PeriodicalId\":7665,\"journal\":{\"name\":\"American Journal of Reproductive Immunology\",\"volume\":\"93 2\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-02-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American Journal of Reproductive Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/aji.70057\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American Journal of Reproductive Immunology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/aji.70057","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Investigation of the Potential Treatment Effect of Botulinum Toxin Type A in Ovarian Torsion: Findings From the Rat Model
Background
The aim of this study was to investigate the effects of botulinum toxin A on histological abnormalities, loss of ovarian reserve, and angiogenesis within the ovarian torsion–detorsion paradigm.
Methods
A total of 45 female Wistar albino rats were used for the study. The rats were randomly divided into five groups (n = 9 in each group): Control, Torsion, Torsion + Detorsion, Torsion + Detorsion + Botulinum toxin, and Botulinum toxin only. Follicular cell degeneration, vascular congestion, hemorrhage, inflammation, and vascular endothelial growth factor (VEGF) expression scores were compared histopathologically between the groups. Follicle numbers were compared. Anti-mullerian hormone levels were measured before and after torsion, detorsion, and treatments.
Results
Follicular cell degeneration, vascular congestion, hemorrhage, inflammation, and VEGF expression differed significantly between groups for each score (p < 0.001). The VEGF expression score was found to increase more in the torsion + detorsion + botulinum toxin group than in the torsion + detorsion group (2.67 ± 0.71 and 2.22 ± 0.67, respectively). There was a significant difference between the groups in secondary follicle counts (p = 0.04). The groups showed statistically significant differences in their anti-mullerian hormone levels (p < 0.001).
Conclusion
This initial investigation of the effects of botulinum toxin on ovarian torsion (OT) has shown that botulinum toxin type A has the potential to be a useful adjunct treatment for fertility preservation. Our results proved the ovarian protective effect of botulinum toxin type A in rats with OT. Therefore, botulinum toxin type A may be a potential preventive agent against ovarian damage caused by torsion in the future.
期刊介绍：
The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.
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