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IF 2.3 4区医学 Q2 DERMATOLOGY

Journal of Cosmetic Dermatology Pub Date : 2025-02-07 DOI:10.1111/jocd.70027

Alina Kilasoniya, Lidia Zabegina, Andrey Shalaev, Anna Artemyeva, Anastasia Malek

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引用次数: 0

摘要

背景本研究探讨了细胞外囊泡（EVs）在皮肤再生和年轻化中的潜力。细胞外小泡是由各种类型细胞释放的纳米级囊泡，在细胞间通信中发挥着至关重要的作用。目的重申血液中的外泌体在细胞间通信中的关键作用及其在皮肤组织再生中的潜力，利用现有研究（包括人体数据），倡导将外泌体作为皮肤健康的一种可行的无细胞疗法。方法该研究采用一种新颖的分离技术，结合 PEG 和右旋糖酐以及超速离心法，从血浆中提取外泌体。包括 NTA、AFM、Cryo-TEM 和 FC 在内的表征技术证实了 EVs 的成功分离和表征。结果研究表明，血液中的 EVs 对小鼠和人体模型中成纤维细胞的增殖、胶原蛋白和弹性蛋白的生成有积极作用。尽管取得了进展，但在获得质量和浓度一致的 EVs 方面仍存在挑战。研究结果证明了 EVs 与皮肤健康的临床相关性，并提出了在皮肤年轻化方面的潜在应用。研究还讨论了未来的研究方向和研究局限性，有助于加深对基于 EVs 的疗法的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Stimulatory Effect of Mammalian Exosomes on Intradermal Fibroblast Proliferation

查看原文本刊更多论文

Stimulatory Effect of Mammalian Exosomes on Intradermal Fibroblast Proliferation

Background

This study investigates the potential of extracellular vesicles (EVs) in skin regeneration and rejuvenation. EVs, nanoscale vesicles released by various cell types, play a crucial role in intercellular communication.

Objective

To reaffirm the pivotal role of blood-derived exosomes in intercellular communication and their potential for skin tissue regeneration, leveraging existing research, including human data, to advocate for exosomes as a viable cell-free therapy for skin health.

Methods

The study employs a novel isolation technique combining PEG and Dextran with ultracentrifugation to extract EVs from plasma. Characterization techniques, including NTA, AFM, Cryo-TEM, and FC, confirm the successful isolation and characterization of EVs.

Results

The study demonstrates positive effects of blood-derived EVs on fibroblast proliferation, collagen, and elastin production in murine and human models. Despite advancements, challenges persist in obtaining consistent EVs quality and concentration. The findings support the clinical relevance of EVs in skin health and suggest potential applications for skin rejuvenation. Future research directions and study limitations are also discussed, contributing to the evolving understanding of EVs-based therapies.

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来源期刊

Journal of Cosmetic Dermatology DERMATOLOGY-

CiteScore

4.30

自引率

13.00%

发文量

818

审稿时长

>12 weeks

期刊介绍： The Journal of Cosmetic Dermatology publishes high quality, peer-reviewed articles on all aspects of cosmetic dermatology with the aim to foster the highest standards of patient care in cosmetic dermatology. Published quarterly, the Journal of Cosmetic Dermatology facilitates continuing professional development and provides a forum for the exchange of scientific research and innovative techniques. The scope of coverage includes, but will not be limited to: healthy skin; skin maintenance; ageing skin; photodamage and photoprotection; rejuvenation; biochemistry, endocrinology and neuroimmunology of healthy skin; imaging; skin measurement; quality of life; skin types; sensitive skin; rosacea and acne; sebum; sweat; fat; phlebology; hair conservation, restoration and removal; nails and nail surgery; pigment; psychological and medicolegal issues; retinoids; cosmetic chemistry; dermopharmacy; cosmeceuticals; toiletries; striae; cellulite; cosmetic dermatological surgery; blepharoplasty; liposuction; surgical complications; botulinum; fillers, peels and dermabrasion; local and tumescent anaesthesia; electrosurgery; lasers, including laser physics, laser research and safety, vascular lasers, pigment lasers, hair removal lasers, tattoo removal lasers, resurfacing lasers, dermal remodelling lasers and laser complications.