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IF 1.7 4区环境科学与生态学 Q3 ECOLOGY

Aquatic Ecology Pub Date : 2024-11-08 DOI:10.1007/s10452-024-10132-3

Yan Sun, Dayong Xu, Xi Chen, Junming Zhou, Changwei Jiang, Zhiqiu Huang, Dongming Qi

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引用次数: 0

摘要

亚硝酸盐在水生生境中的积累会诱发毒性并导致鱼类脾脏损伤。本研究通过转录组分析确定了参与大口鲈鱼脾脏对亚硝酸盐应激反应的叉头盒（FOX）转录因子。系统发生分析将 93 个 FOX 基因分为 17 个亚组，其中 FOXN、FOXP 和 FOXO 在亚硝酸盐处理后出现显著上调。进一步分析发现，msFOX_29、msFOX_81、msFOX_89和msFOX_7是介导脾脏损伤的关键FOX转录调控因子。共表达网络分析显示，这些 FOX 转录因子可能调控涉及凋亡（CASP8）、免疫细胞浸润（CD34）、蛋白质合成（UBA52、FARSB）、信号转导（PPP2CB）和新陈代谢（GUK1）等通路的下游靶标。这些靶标的启动子区域都含有 FOX 结合位点，并在亚硝酸盐胁迫下上调。此外，研究还发现 FOX 的表达与免疫细胞浸润的变化有关。跨物种分析揭示了 FOXN、FOXP 和 FOXO 亚群在更多鱼类脾脏反应中的系统发育保守性和功能重要性。我们的研究描绘了介导大口鲈亚硝酸盐诱导的脾脏损伤的 FOX 转录调控网络，为了解亚硝酸盐在鱼类中的毒性机制提供了见解。

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Deciphering the transcriptional regulatory networks of FOX genes in nitrite-induced spleen injury in largemouth bass

Nitrite accumulation in aquatic habitats can induce toxicity and cause spleen injury in fish. This study identified forkhead box (FOX) transcription factors involved in the splenic response to nitrite stress in largemouth bass through transcriptomic analysis. Phylogenetic analysis classified 93 FOX genes into 17 subgroups, with FOXN, FOXP and FOXO showing significant upregulation after nitrite treatment. Further analysis identified msFOX_29, msFOX_81, msFOX_89 and msFOX_7 as key FOX transcriptional regulators mediating spleen injury. Co-expression network analysis revealed that these FOX transcription factors may regulate downstream targets involved in pathways such as apoptosis (CASP8), immune cell infiltration (CD34), protein synthesis (UBA52, FARSB), signal transduction (PPP2CB), and metabolism (GUK1). These targets contained FOX binding sites in promoter regions and were upregulated under nitrite stress. Additionally, FOX expression was found correlated with changes in immune cell infiltration. Cross-species analyses revealed phylogenetic conservation and functional importance of FOXN, FOXP and FOXO subgroups in the splenic responses more fish species. Our study delineates the FOX transcriptional regulatory networks mediating nitrite-induced spleen injury in largemouth bass and provides insights into nitrite toxicity mechanisms in fish.

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来源期刊

Aquatic Ecology 环境科学-海洋与淡水生物学

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Aquatic Ecology publishes timely, peer-reviewed original papers relating to the ecology of fresh, brackish, estuarine and marine environments. Papers on fundamental and applied novel research in both the field and the laboratory, including descriptive or experimental studies, will be included in the journal. Preference will be given to studies that address timely and current topics and are integrative and critical in approach. We discourage papers that describe presence and abundance of aquatic biota in local habitats as well as papers that are pure systematic. The journal provides a forum for the aquatic ecologist - limnologist and oceanologist alike- to discuss ecological issues related to processes and structures at different integration levels from individuals to populations, to communities and entire ecosystems.