含α -蒎烯、柠檬醛、樟脑和百里香酚纳米脂质体对埃及伊蚊和斯氏按蚊的杀蚊效果

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-02-07 DOI:10.1007/s11686-025-00993-5

Alireza Sanei-Dehkordi, Narges Fereydouni, Mahmoud Agholi, Seyed AhmadReza Ziaei, Zahra Azadpour, Elham Zarenezhad, Mahmoud Osanloo

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引用次数: 0

摘要

背景：由埃及伊蚊或斯氏按蚊传播的疾病，如寨卡病毒、登革热和疟疾，对公共卫生构成重大风险，特别是在热带地区。植物源化合物由于其固有的安全性和对蚊子的潜在有效性而成为有希望的替代品。本研究旨在通过开发纳米脂质体配方来提高某些天然化合物（包括α-蒎烯、柠檬醛、樟脑和百里香酚）的功效。方法采用乙醇注射法制备含有α-蒎烯、柠檬醛、樟脑和百里酚的纳米脂质体，并对其进行表征。根据世卫组织推荐的指南，调查了它们对埃及伊蚊和斯氏按蚊的杀幼虫效果。结果纳米脂质体粒径分别为105±7、86±5、149±5和135±8 nm， zeta电位分别为- 25.1±0.5、-17.2±1.2、-16.4±1.6和- 21.3±1.7 mV。此外，ATR-FTIR（衰减全反射-傅里叶变换红外）分析验证了化合物的成功加载。纳米脂质体化合物在杀幼虫生物测定中表现出比其非配方对应物更优越的性能。含百里酚的纳米脂质体对Ae的致死浓度50 （LC50）为20µg/mL。蚊。含有柠檬醛的纳米脂质体对紫杉醇的LC50为20µg/mL。stephensi。结论纳米脂质体是提高植物基杀幼虫剂药效的有效载体。这可能在制定可持续的蚊虫控制战略方面发挥重要作用。

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Larvicidal Efficacies of Nanoliposomes Containing Alpha-pinene, Citral, Camphor, and Thymol Against Aedes aegypti and Anopheles stephensi Mosquito Vectors

Background

Diseases transmitted by Aedes aegypti or Anopheles stephensi, such as Zika virus, dengue fever, and malaria, pose substantial risks to public health, particularly in tropical areas. Plant-derived compounds have emerged as promising alternatives due to their inherent safety and potential efficiency against mosquitoes. This study aimed to improve the efficacy of certain natural compounds, including α-pinene, citral, camphor, and thymol, by developing nanoliposomal formulations.

Methods

The ‎nanoliposomes containing α-pinene, citral, camphor, and thymol were prepared using the ethanol injection method and then characterized. Using WHO-recommended guidelines, their larvicidal efficacy was investigated against Aedes aegypti and Anopheles stephensi.

Results

The ‎nanoliposomes particle sizes were 105 ± 7, 86 ± 5, 149 ± 5, and 135 ± 8 nm, and zeta potentials ‎were − 25.1 ± 0.5, -17.2 ± 1.2, -16.4 ± 1.6, and − 21.3 ± 1.7 mV, respectively. In addition, the ATR-FTIR (Attenuated Total Reflectance-Fourier Transform InfraRed) analysis verified the successful loading of the compound. Nanoliposomal compounds exhibited superior performance compared to their non-formulated counterparts in larvicidal bioassays. The nanoliposomes containing thymol showed the highest efficacy, with a Lethal Concentration 50 (LC₅₀) of 20 µg/mL against Ae. aegypti. Nanoliposomes containing citral exhibited an LC₅₀ of 20 µg/mL against An. stephensi.

Conclusions

The results suggest that nanoliposomes have the potential to serve as an effective vehicle to improve the efficiency of plant-based larvicides. This could play a significant role in developing sustainable mosquito control strategies.

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.