体外启动STING信号通路可增强乙型肝炎疫苗诱导的树突状细胞的成熟和活化

IF 3.3 4区 医学 Q3 IMMUNOLOGY
Chaomin Ren , Xufeng Cui , Huixin Wang , Cong Jin , Linying Gao , Yandi Li , Weigang Wang , Tian Yao , Demei Zhang , Yongliang Feng , Keke Wang , Suping Wang
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引用次数: 0

摘要

本研究探讨了STING信号通路在乙型肝炎疫苗应答中增强树突状细胞(dc)成熟和激活中的作用。通过分析GSE52894数据集,我们比较了成熟树突状细胞(mDCs)和未成熟树突状细胞(iDCs)之间的差异表达基因。在体外,用STING激动剂2’3’-cGAMP单独或与脂多糖(LPS)或乙肝疫苗联合治疗iDCs,评估STING通路中共刺激分子和关键信号分子(包括STING、pNF-κBp65和pIRF3)的表达。结果表明,与iDCs相比,mDCs表达的STING mRNA水平显著升高(P <;0.01)。2’3’-cGAMP处理增加了STING的表达,激活了下游信号分子pNF-κBp65和pIRF3。2 ' 3 ' -cGAMP和LPS联合处理能比单独处理更有效地上调共刺激分子(CD80、CD86、HLA-DR、CD11c) (P <;0.05)。2 ' 3 ' -cGAMP与乙型肝炎疫苗联合治疗可显著提高共刺激分子的表达。此外,2 ' 3 ' -cGAMP与乙肝疫苗联合治疗可增强STING、pNF-κBp65和pIRF3的表达。混合淋巴细胞反应试验表明,2 ' 3 ' -cGAMP与乙肝疫苗联合治疗组对CD4+T细胞增殖的影响明显强于单纯疫苗治疗组。总之,2’3’-cGAMP通过激活STING/IRF3和STING/NF-κB通路,促进dc成熟和CD4+T细胞增殖,从而响应乙肝疫苗,突出了其作为佐剂提高疫苗效力的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In virto priming of the STING signaling pathway enhances the maturation and activation of dendritic cells induced by hepatitis B vaccine

In virto priming of the STING signaling pathway enhances the maturation and activation of dendritic cells induced by hepatitis B vaccine
This study investigates the role of the STING signaling pathway in enhancing dendritic cells (DCs) maturation and activation in response to the hepatitis B vaccine. By analyzing the GSE52894 dataset, we compared differentially expressed genes between mature dendritic cells (mDCs) and immature dendritic cells (iDCs). In vitro, iDCs were treated with the STING agonist 2′3′-cGAMP, either alone or in combination with lipopolysaccharide (LPS) or the hepatitis B vaccine, to assess the expression of costimulatory molecules and key signaling molecules in the STING pathway, including STING, pNF-κBp65, and pIRF3. The results indicated that mDCs expressed significantly higher levels of STING mRNA compared to iDCs (P < 0.01). Treatment with 2′3′-cGAMP increased STING expression and activated downstream signaling molecules pNF-κBp65 and pIRF3. Co-treatment with 2′3′-cGAMP and LPS upregulated costimulatory molecules (CD80, CD86, HLA-DR, CD11c) more effectively than LPS alone (P < 0.05). Co-treatment with 2′3′-cGAMP and the hepatitis B vaccine resulted in significantly higher expression of costimulatory molecules compared to vaccine-only treatment. Furthermore, co-treatment with 2′3′-cGAMP and the hepatitis B vaccine enhanced STING, pNF-κBp65, and pIRF3 expression relative to vaccine alone. Mixed lymphocyte reaction assays demonstrated that the 2′3′-cGAMP and hepatitis B vaccine co-treatment group had a significantly stronger effect on the proliferation of CD4+T cells compared to the vaccine-only treatment group. In conclusion, 2′3′-cGAMP enhances DCs maturation and promotes CD4+T cells proliferation in response to the hepatitis B vaccine by activating the STING/IRF3 and STING/NF-κB pathways, highlighting its potential as an adjuvant to improve vaccine efficacy.
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来源期刊
Immunology letters
Immunology letters 医学-免疫学
CiteScore
7.60
自引率
0.00%
发文量
86
审稿时长
44 days
期刊介绍: Immunology Letters provides a vehicle for the speedy publication of experimental papers, (mini)Reviews and Letters to the Editor addressing all aspects of molecular and cellular immunology. The essential criteria for publication will be clarity, experimental soundness and novelty. Results contradictory to current accepted thinking or ideas divergent from actual dogmas will be considered for publication provided that they are based on solid experimental findings. Preference will be given to papers of immediate importance to other investigators, either by their experimental data, new ideas or new methodology. Scientific correspondence to the Editor-in-Chief related to the published papers may also be accepted provided that they are short and scientifically relevant to the papers mentioned, in order to provide a continuing forum for discussion.
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