牛输卵管上皮细胞裂解液对牛体外受精胚胎发育能力和质量的影响

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-02-03 DOI:10.1016/j.theriogenology.2025.02.002

Funa Ota , Hayato Minowa , Rina Miura , Tetsuma Murase , Tokunori Yamamoto , Takehiro Himaki

{"title":"牛输卵管上皮细胞裂解液对牛体外受精胚胎发育能力和质量的影响","authors":"Funa Ota , Hayato Minowa , Rina Miura , Tetsuma Murase , Tokunori Yamamoto , Takehiro Himaki","doi":"10.1016/j.theriogenology.2025.02.002","DOIUrl":null,"url":null,"abstract":"<div><div>In vitro fertilization (IVF) technology for embryo production has been applied in basic research, animal husbandry and medicine. However, the developmental efficiency and quality of embryos produced by IVF are inferior to those produced in vivo. In this study, we investigated the effects of supplementing bovine oviductal epithelial cells (BOEC) lysate during the in vitro culture period on the developmental competence and quality of bovine embryos. IVF embryos were cultured for 4 days post-IVF in medium supplemented with 10 % BOEC lysate at various concentrations (1.0 × 105, 2.0 × 105, and 4.0 × 105 cells/mL) or 10 % PBS (−), which was used to adjust the lysate concentration (control). BOEC lysate at 2.0 × 105 cells/mL significantly increased the blastocyst formation rate compared to that in the control group. Blastocysts from BOEC lysate supplemented groups showed significantly lower apoptosis rate than that in the control group. The ratio of inner cell mass cell number in blastocysts was significantly higher in all BOEC lysate supplemented groups than in the control group. The survival rate after vitrification/thawing was improved in the 1.0 × 105 and 2.0 × 105 cells/mL BOEC lysate supplemented groups. In addition, gene expression analysis of blastocysts showed that 2.0 × 105 cells/mL of BOEC lysate supplementation significantly enhanced the expression of anti-apoptotic genes (BCL2 and BIRC5), antioxidant-related genes (GPX1 and SOD2), and cell differentiation-related genes (SOX2 and OCT4). In conclusion, supplementation with 2.0 × 105 cells/mL BOEC lysate during early in vitro culture improved the developmental competence and quality of bovine IVF embryos.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"236 ","pages":"Pages 96-104"},"PeriodicalIF":2.4000,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of bovine oviductal epithelial cell lysate on the developmental competence and quality of bovine in vitro fertilized embryos\",\"authors\":\"Funa Ota , Hayato Minowa , Rina Miura , Tetsuma Murase , Tokunori Yamamoto , Takehiro Himaki\",\"doi\":\"10.1016/j.theriogenology.2025.02.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In vitro fertilization (IVF) technology for embryo production has been applied in basic research, animal husbandry and medicine. However, the developmental efficiency and quality of embryos produced by IVF are inferior to those produced in vivo. In this study, we investigated the effects of supplementing bovine oviductal epithelial cells (BOEC) lysate during the in vitro culture period on the developmental competence and quality of bovine embryos. IVF embryos were cultured for 4 days post-IVF in medium supplemented with 10 % BOEC lysate at various concentrations (1.0 × 105, 2.0 × 105, and 4.0 × 105 cells/mL) or 10 % PBS (−), which was used to adjust the lysate concentration (control). BOEC lysate at 2.0 × 105 cells/mL significantly increased the blastocyst formation rate compared to that in the control group. Blastocysts from BOEC lysate supplemented groups showed significantly lower apoptosis rate than that in the control group. The ratio of inner cell mass cell number in blastocysts was significantly higher in all BOEC lysate supplemented groups than in the control group. The survival rate after vitrification/thawing was improved in the 1.0 × 105 and 2.0 × 105 cells/mL BOEC lysate supplemented groups. In addition, gene expression analysis of blastocysts showed that 2.0 × 105 cells/mL of BOEC lysate supplementation significantly enhanced the expression of anti-apoptotic genes (BCL2 and BIRC5), antioxidant-related genes (GPX1 and SOD2), and cell differentiation-related genes (SOX2 and OCT4). In conclusion, supplementation with 2.0 × 105 cells/mL BOEC lysate during early in vitro culture improved the developmental competence and quality of bovine IVF embryos.</div></div>\",\"PeriodicalId\":23131,\"journal\":{\"name\":\"Theriogenology\",\"volume\":\"236 \",\"pages\":\"Pages 96-104\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-02-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Theriogenology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0093691X25000482\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"REPRODUCTIVE BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theriogenology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0093691X25000482","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

体外受精（IVF）技术在基础研究、畜牧业和医学等领域都有广泛的应用。然而，体外受精产生的胚胎的发育效率和质量不如体内产生的胚胎。本研究研究了在体外培养过程中添加牛输卵管上皮细胞（BOEC）裂解液对牛胚胎发育能力和质量的影响。体外受精后，试管婴儿胚胎在培养基中培养4天，培养基中添加10%不同浓度的BOEC裂解液（1.0 × 105、2.0 × 105和4.0 × 105细胞/mL）或10% PBS (-)， PBS用于调节裂解液浓度（对照）。2.0 × 105个细胞/mL的BOEC裂解液与对照组相比显著提高囊胚形成率。BOEC裂解液添加组囊胚凋亡率显著低于对照组。各添加组囊胚内细胞质量细胞数比均显著高于对照组。1.0 × 105和2.0 × 105细胞/mL BOEC裂解液添加组玻璃化/解冻后的存活率提高。此外，胚泡基因表达分析显示，添加2.0 × 105个细胞/mL BOEC裂解物可显著提高抗凋亡基因（BCL2和BIRC5）、抗氧化相关基因（GPX1和SOD2）和细胞分化相关基因（SOX2和OCT4）的表达。结果表明，体外培养早期添加2.0 × 105个细胞/mL BOEC裂解物可提高牛体外受精胚胎的发育能力和质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Effect of bovine oviductal epithelial cell lysate on the developmental competence and quality of bovine in vitro fertilized embryos

In vitro fertilization (IVF) technology for embryo production has been applied in basic research, animal husbandry and medicine. However, the developmental efficiency and quality of embryos produced by IVF are inferior to those produced in vivo. In this study, we investigated the effects of supplementing bovine oviductal epithelial cells (BOEC) lysate during the in vitro culture period on the developmental competence and quality of bovine embryos. IVF embryos were cultured for 4 days post-IVF in medium supplemented with 10 % BOEC lysate at various concentrations (1.0 × 10⁵, 2.0 × 10⁵, and 4.0 × 10⁵ cells/mL) or 10 % PBS (−), which was used to adjust the lysate concentration (control). BOEC lysate at 2.0 × 10⁵ cells/mL significantly increased the blastocyst formation rate compared to that in the control group. Blastocysts from BOEC lysate supplemented groups showed significantly lower apoptosis rate than that in the control group. The ratio of inner cell mass cell number in blastocysts was significantly higher in all BOEC lysate supplemented groups than in the control group. The survival rate after vitrification/thawing was improved in the 1.0 × 10⁵ and 2.0 × 10⁵ cells/mL BOEC lysate supplemented groups. In addition, gene expression analysis of blastocysts showed that 2.0 × 10⁵ cells/mL of BOEC lysate supplementation significantly enhanced the expression of anti-apoptotic genes (BCL2 and BIRC5), antioxidant-related genes (GPX1 and SOD2), and cell differentiation-related genes (SOX2 and OCT4). In conclusion, supplementation with 2.0 × 10⁵ cells/mL BOEC lysate during early in vitro culture improved the developmental competence and quality of bovine IVF embryos.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.