Spectrofluorimetric and theoretical investigation of a glucocorticoid drug with HSA: Quantitative studies in real sample

For the assay of methylprednisolone (MP) in pharmaceutical formulation, a fluorescence-based spectroscopic approach is developed using the protein, human serum albumin (HSA). The structural alterations in human serum albumin (HSA) following its interaction with methylprednisolone (MP) (10–32 μg mL⁻¹) were determined by spectroscopy, which included the use of fluorescence and synchronous fluorescence. The calibration curve was modeled using the fluorescence method. The devised approach has a limit of detection of 1.16 μg mL⁻¹ and a limit of quantification of 3.535 μg mL⁻¹ for MP estimation that is straightforward, sensitive, accurate, and selective. The reference approach and the suggested method were contrasted to show which was more appropriate for MP quality control in its dosage forms. The recovery data obtained from 99.63% to 100.0% for intra-day and 99.60–100.1%, for inter-day precision. The developed method showed remarkable sensitivity to dosage forms and real sample (urine), suggesting possible application, when applied to dose forms with a relative standard deviation of less than 2%.