基于Au/PDA/AuNps共修饰PCF的灵敏度增强SPR/LSPR生物传感器检测兔IgG

IF 2.6 3区计算机科学 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC

Optical Fiber Technology Pub Date : 2025-02-03 DOI:10.1016/j.yofte.2025.104148

Zhiyang Wang, Ailing Zhang, Pengxiang Chang, Yanmei Shi, Zhen Li

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引用次数: 0

摘要

提出了一种由金膜、聚多巴胺（PDA）和金纳米粒子（AuNps）共改性的基于光子晶体光纤（PCF）的SPR生物传感器。利用Au薄膜激发的SPR与AuNps激发的局域表面等离子体共振（LSPR）耦合以及PDA的高折射率，提高了传感器的折射率灵敏度。实验结果表明，PCF-Au- pda - aunps传感器在1.335 ~ 1.365的RI范围内获得了3523.10 nm/RIU的高RI灵敏度，是PCF-Au传感器的2.07倍。此外，为了对兔IgG进行生物检测，在AuNps表面修饰一层具有良好粘附性和生物相容性的PDA，以固定一层山羊抗兔免疫球蛋白G（IgG）。该传感器对兔IgG的生物灵敏度为0.951 nm/（µg/mL），检出限（LOD）为0.021µg/mL。由于其良好的生物传感性能，这种基于SPR-LSPR耦合的光纤生物传感器适合应用于临床疾病诊断和免疫测定。

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Sensitivity enhanced SPR/LSPR biosensor based on Au/PDA/AuNps co-modified PCF for rabbit IgG detection

A photonic crystal fiber (PCF)-based SPR biosensor co-modified by Au film, polydopamine (PDA) and Au nanoparticles (AuNps) is proposed. The refractive index(RI) sensitivity of the sensor is enhanced by the coupling between the SPR inspired in the Au film and the localized surface plasmon resonance (LSPR) inspired in AuNps, as well as the high RI of the PDA. The experimental results show that the PCF-Au-PDA-AuNps sensor obtains a high RI sensitivity of 3523.10 nm/RIU in the RI range of 1.335–––1.365, which is 2.07 times of that of the PCF-Au sensor. In addition, in order to bioassay to rabbit IgG, an extra layer of PDA with excellent adhesion and biocompatibility properties is modified on the surface of AuNps to anchor a layer of goat anti-rabbit Immunoglobulin G(IgG). The bio-sensitivity of the sensor for rabbit IgG reaches 0.951 nm/(µg/mL), and the limit of detection (LOD) is 0.021 µg/mL. Due to its good biosensing performance, this fiber-optic biosensor based on SPR-LSPR coupling is suitable to be applied in clinical disease diagnosis and immunoassay.

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来源期刊

Optical Fiber Technology 工程技术-电信学

CiteScore

4.80

自引率

11.10%

发文量

327

审稿时长

63 days

期刊介绍： Innovations in optical fiber technology are revolutionizing world communications. Newly developed fiber amplifiers allow for direct transmission of high-speed signals over transcontinental distances without the need for electronic regeneration. Optical fibers find new applications in data processing. The impact of fiber materials, devices, and systems on communications in the coming decades will create an abundance of primary literature and the need for up-to-date reviews. Optical Fiber Technology: Materials, Devices, and Systems is a new cutting-edge journal designed to fill a need in this rapidly evolving field for speedy publication of regular length papers. Both theoretical and experimental papers on fiber materials, devices, and system performance evaluation and measurements are eligible, with emphasis on practical applications.