RAA-CRISPR-Cas12a快速灵敏检测鸡毛滴虫

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-02-01 DOI:10.1016/j.vetpar.2025.110412

Yuhan Zhou , Yaqian Chen , Xinglong Song , Zhenyu Zhong , Qingyun Guo , Shengfan Jing , Olalekan Opeyemi Ayanniyi , Zhenxiao Lu , Qingxun Zhang , Congshan Yang

{"title":"RAA-CRISPR-Cas12a快速灵敏检测鸡毛滴虫","authors":"Yuhan Zhou , Yaqian Chen , Xinglong Song , Zhenyu Zhong , Qingyun Guo , Shengfan Jing , Olalekan Opeyemi Ayanniyi , Zhenxiao Lu , Qingxun Zhang , Congshan Yang","doi":"10.1016/j.vetpar.2025.110412","DOIUrl":null,"url":null,"abstract":"<div><div><em>Trichomonas gallinae</em> (<em>T. gallinae</em>) is an important pathogen causing trichomoniasis in birds, especially pigeons. Rapid and sensitive detection methods for <em>T. gallinae</em> are urgently needed to diagnose <em>T. gallinae</em> early to reduce poultry industry losses. Therefore, we developed a rapid and sensitive diagnostic method based on recombinase-aided amplification (RAA) assay and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 12a (CRISPR/Cas12a) system to detect <em>T. gallinae</em>. The RAA-CRISPR/Cas12a method can be divided into RAA-CRISPR/Cas12a fluorescent signal (RAA-CRISPR/Cas12a-FL) and RAA-CRISPR/Cas12a lateral flow strip (RAA-CRISPR/Cas12-LFS). Both RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12-LFS methods show the property of rapid. sensitive, and does not require a sophisticated instrument, and they allow the detection of <em>T. gallinae</em> in less than 1 hr. Meanwhile, they have satisfactory specificity and can accurately detect <em>T. gallinae</em> in samples of different pathogens. In summary, the RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12-LFS methods we constructed can be used for on-site <em>T. gallinae</em> detection and resource-poor areas.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"Article 110412"},"PeriodicalIF":2.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rapid and sensitive detection of Trichomonas gallinae using RAA-CRISPR-Cas12a\",\"authors\":\"Yuhan Zhou , Yaqian Chen , Xinglong Song , Zhenyu Zhong , Qingyun Guo , Shengfan Jing , Olalekan Opeyemi Ayanniyi , Zhenxiao Lu , Qingxun Zhang , Congshan Yang\",\"doi\":\"10.1016/j.vetpar.2025.110412\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Trichomonas gallinae</em> (<em>T. gallinae</em>) is an important pathogen causing trichomoniasis in birds, especially pigeons. Rapid and sensitive detection methods for <em>T. gallinae</em> are urgently needed to diagnose <em>T. gallinae</em> early to reduce poultry industry losses. Therefore, we developed a rapid and sensitive diagnostic method based on recombinase-aided amplification (RAA) assay and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 12a (CRISPR/Cas12a) system to detect <em>T. gallinae</em>. The RAA-CRISPR/Cas12a method can be divided into RAA-CRISPR/Cas12a fluorescent signal (RAA-CRISPR/Cas12a-FL) and RAA-CRISPR/Cas12a lateral flow strip (RAA-CRISPR/Cas12-LFS). Both RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12-LFS methods show the property of rapid. sensitive, and does not require a sophisticated instrument, and they allow the detection of <em>T. gallinae</em> in less than 1 hr. Meanwhile, they have satisfactory specificity and can accurately detect <em>T. gallinae</em> in samples of different pathogens. In summary, the RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12-LFS methods we constructed can be used for on-site <em>T. gallinae</em> detection and resource-poor areas.</div></div>\",\"PeriodicalId\":23716,\"journal\":{\"name\":\"Veterinary parasitology\",\"volume\":\"334 \",\"pages\":\"Article 110412\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary parasitology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0304401725000238\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304401725000238","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

鸡滴虫（T. gallinae）是引起鸟类特别是鸽子滴虫病的重要病原体。迫切需要快速、灵敏的鸡糜泻检测方法来早期诊断鸡糜泻，以减少家禽业的损失。为此，我们建立了一种基于重组酶辅助扩增（RAA）技术、短回文重复序列（CRISPR）和CRISPR相关蛋白12a （CRISPR/Cas12a）系统聚类的快速、灵敏的鸡绦虫检测方法。RAA-CRISPR/Cas12a方法可分为RAA-CRISPR/Cas12a荧光信号（RAA-CRISPR/Cas12a- fl）和RAA-CRISPR/Cas12a侧流条带（RAA-CRISPR/Cas12-LFS）。RAA-CRISPR/Cas12a-FL和RAA-CRISPR/Cas12-LFS方法均表现出快速的特性。灵敏，不需要复杂的仪器，它们可以在不到1 hr的时间内检测出鸡瘿杆菌。同时，它们具有良好的特异性，能准确检测出不同病原菌样品中的鸡绦虫。综上所述，我们构建的RAA-CRISPR/Cas12a-FL和RAA-CRISPR/Cas12-LFS方法可用于鸡绦虫现场检测和资源贫乏地区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Rapid and sensitive detection of Trichomonas gallinae using RAA-CRISPR-Cas12a

Trichomonas gallinae (T. gallinae) is an important pathogen causing trichomoniasis in birds, especially pigeons. Rapid and sensitive detection methods for T. gallinae are urgently needed to diagnose T. gallinae early to reduce poultry industry losses. Therefore, we developed a rapid and sensitive diagnostic method based on recombinase-aided amplification (RAA) assay and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 12a (CRISPR/Cas12a) system to detect T. gallinae. The RAA-CRISPR/Cas12a method can be divided into RAA-CRISPR/Cas12a fluorescent signal (RAA-CRISPR/Cas12a-FL) and RAA-CRISPR/Cas12a lateral flow strip (RAA-CRISPR/Cas12-LFS). Both RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12-LFS methods show the property of rapid. sensitive, and does not require a sophisticated instrument, and they allow the detection of T. gallinae in less than 1 hr. Meanwhile, they have satisfactory specificity and can accurately detect T. gallinae in samples of different pathogens. In summary, the RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12-LFS methods we constructed can be used for on-site T. gallinae detection and resource-poor areas.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.