一种紧凑的棱镜显微镜,用于液体活检的高灵敏度测量。

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS
Laura Perego, Caterina Dallari, Chiara Falciani, Alessandro Pini, Lucia Gardini, Caterina Credi, Francesco Saverio Pavone
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引用次数: 0

摘要

对敏感、便携和负担得起的疾病检测方法的需求日益增长,刺激了用于快速早期诊断、人口大规模筛查和床上监测的先进生物传感器的发展。目前的高灵敏度器件面临着生产成本高和多路信号检测等障碍。为了解决这些问题,我们开发了一种基于棱镜的全内反射系统,该系统与金纳米颗粒的表面功能化技术相结合,能够对合成和人体液体样品中的特定分析物进行高灵敏度和快速的倏逝波散射检测。为了验证其有效性,我们在合成和人血清样本中进行了散射实验,利用功能化的AuNPs识别细菌脂多糖作为败血症的生物标志物。我们证明了这种特定病理生物标志物在每毫升浓度范围内的飞图的显著敏感性。基于这一结果,我们设想我们的液体活检技术在临床应用的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A Compact Prism-Based Microscope for Highly Sensitive Measurements in Fluid Biopsy

A Compact Prism-Based Microscope for Highly Sensitive Measurements in Fluid Biopsy

The increasing demand for sensitive, portable, and affordable disease detection methods has spurred the development of advanced biosensors for rapid early-stage diagnosis, population mass screening, and bed-monitoring. Current high-sensitivity devices face hurdles such as high production costs and challenges in multiplexed signal detection. To address these, we developed a prism-based total internal reflection system which, in combination with surface functionalization techniques of gold nanoparticles, enables evanescent wave scattering for highly sensitive and rapid detection of specific analytes in both synthetic and human liquid samples. To validate its efficacy, we conducted scattering experiments in synthetic and human serum samples, exploiting functionalized AuNPs to recognize bacterial lipopolysaccharides as biomarkers for sepsis disease. We demonstrate a remarkable sensitivity in the femtogram per mL concentration range for this specific pathological biomarker. Based on this result we envisage the potential adoption of our technique for liquid biopsy in the clinical scenario.

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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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