不寻常的怀疑：中间丝蛋白在线粒体形态中的新作用。

IF 4.5 3区生物学 Q2 CELL BIOLOGY

Mitochondrion Pub Date : 2025-02-03 DOI:10.1016/j.mito.2025.102008

Irene M.G.M. Hemel , Carlijn Steen , Simon L.I.J. Denil , Gökhan Ertaylan , Martina Kutmon , Michiel Adriaens , Mike Gerards

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引用次数: 0

摘要

线粒体动力学对细胞稳态至关重要。然而，并不是所有的蛋白质都是已知的。利用蛋白相互作用（PPI）方法，我们确定了ITPRIPL2参与线粒体动力学。ITPRIPL2与中间丝蛋白vimentin共定位，得到了蛋白质模拟的支持。ITPRIPL2敲低揭示了线粒体伸长，破坏了波形蛋白加工，中间丝的形成，并改变了波形蛋白相关的途径。有趣的是，vimentin敲低也会导致线粒体伸长。这些发现强调了ITPRIPL2是中间丝结构所必需的vimentin相关蛋白，并提示中间丝在线粒体形态中的作用。我们的研究表明，PPI分析是鉴定新的线粒体动力学蛋白的有力方法。

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The unusual suspect: A novel role for intermediate filament proteins in mitochondrial morphology

Mitochondrial dynamics is crucial for cellular homeostasis. However, not all proteins involved are known. Using a protein–protein interaction (PPI) approach, we identified ITPRIPL2 for involvement in mitochondrial dynamics. ITPRIPL2 co-localizes with intermediate filament protein vimentin, supported by protein simulations. ITPRIPL2 knockdown reveals mitochondrial elongation, disrupts vimentin processing, intermediate filament formation, and alters vimentin-related pathways. Interestingly, vimentin knockdown also leads to mitochondrial elongation. These findings highlight ITPRIPL2 as vimentin-associated protein essential for intermediate filament structure and suggest a role for intermediate filaments in mitochondrial morphology. Our study demonstrates that PPI analysis is a powerful approach for identifying novel mitochondrial dynamics proteins.

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来源期刊

Mitochondrion 生物-细胞生物学

CiteScore

9.40

自引率

4.50%

发文量

审稿时长

13.6 weeks

期刊介绍： Mitochondrion is a definitive, high profile, peer-reviewed international research journal. The scope of Mitochondrion is broad, reporting on basic science of mitochondria from all organisms and from basic research to pathology and clinical aspects of mitochondrial diseases. The journal welcomes original contributions from investigators working in diverse sub-disciplines such as evolution, biophysics, biochemistry, molecular and cell biology, genetics, pharmacology, toxicology, forensic science, programmed cell death, aging, cancer and clinical features of mitochondrial diseases.