{"title":"非致病性霍乱弧菌胃肠道毒力基因的出现和检测:对非洲发展中地区公共卫生和水安全的影响","authors":"Bright Esegbuyota Igere","doi":"10.1002/med4.88","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Water, an indispensable component of life, has in recent times been associated with the distribution/spread of gastro-enteric diseases especially as safe water is a concern in Southern, Eastern and Western African developmental regions.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>This study evaluated the molecular fingerprints of potential gastro-enteric associated virulence genes of 58 somatic antigen non-agglutinating <i>Vibrio cholerae</i> 1/139 (<i>SA-NAG-Vc-1/139</i>) strains from domestic water sources. Strains were isolated and characterized applying both culture-based-microbiological techniques and molecular-fingerprinting of target-specific identification genes using simplex/multiplex PCR assay and ERIC-PCR fingerprints.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Our study revealed diverse gene-based indices vis <i>T3SS</i> (29/58, 50.00%), <i>T6SS</i> (33/58, 56.9%), <i>rtxA</i> (37/58, 63.79%), <i>rtxC</i> (12/58, 20.69%), <i>NAG-stn/sto</i> (13/58, 22.41%), <i>prtV</i> (17/58, 29.31%), <i>hlyA</i> (41/58, 70.69%), <i>nanH</i> (40/58, 68.97%), <i>mshA</i> (44/58, 75.86%), <i>chxA</i> (37/58, 63.79%), <i>hapA</i> (17/58, 29.31%), <i>ace</i> (22/58, 37.93%), and <i>cep</i> (20/58, 34.48%) etc. Such results show that 53.45% (31/58) of isolates harboured more than three virulence associated genes while <i>nanH</i>, <i>mshA</i>, <i>chxA</i>, <i>T6SS</i>, <i>T3SS</i>, <i>rtxA</i>, <i>hlyA</i>, <i>mshA</i>, <i>chxA</i>, <i>ace</i> and <i>cep</i> fingerprints were detected predominantly with corresponding ilea-loop test positive strains. ERIC-PCR also showed multiple target specific repetitive intergenic consensus sequence regions ranging from 2 to 8.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>This is an indication that the previously known non-pathogenic strains now harbour potential gastro-enteric virulence which may be controlled by more than one virulent gene dynamics. It also suggests a current potential shift in the virulence dynamics of <i>V</i>. <i>cholerae</i> strains recovered from the study area and a re-evaluated view of the previously non-pathogenic <i>V</i>. <i>cholerae</i> strains. Furthermore, the presence of such genes in <i>SA-NAG-Vc-1/139</i> strains indicates a potential public health related concern. Although these detected potential gastro-enteric associated genes may be implicated in sporadic gastroenteritis, our result has re-emphasized their probable public health concern as they may be involved in both endemic and severe gastroenteritis cases, which suggests the need for water routine monitoring or surveillance.</p>\n </section>\n </div>","PeriodicalId":100913,"journal":{"name":"Medicine Advances","volume":"2 4","pages":"361-374"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/med4.88","citationCount":"0","resultStr":"{\"title\":\"Emergence and detection of gastro-enteric virulence genes in non-pathogenic Vibrio cholerae: Implications for public health and water safety in African developmental regions\",\"authors\":\"Bright Esegbuyota Igere\",\"doi\":\"10.1002/med4.88\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Water, an indispensable component of life, has in recent times been associated with the distribution/spread of gastro-enteric diseases especially as safe water is a concern in Southern, Eastern and Western African developmental regions.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>This study evaluated the molecular fingerprints of potential gastro-enteric associated virulence genes of 58 somatic antigen non-agglutinating <i>Vibrio cholerae</i> 1/139 (<i>SA-NAG-Vc-1/139</i>) strains from domestic water sources. Strains were isolated and characterized applying both culture-based-microbiological techniques and molecular-fingerprinting of target-specific identification genes using simplex/multiplex PCR assay and ERIC-PCR fingerprints.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Our study revealed diverse gene-based indices vis <i>T3SS</i> (29/58, 50.00%), <i>T6SS</i> (33/58, 56.9%), <i>rtxA</i> (37/58, 63.79%), <i>rtxC</i> (12/58, 20.69%), <i>NAG-stn/sto</i> (13/58, 22.41%), <i>prtV</i> (17/58, 29.31%), <i>hlyA</i> (41/58, 70.69%), <i>nanH</i> (40/58, 68.97%), <i>mshA</i> (44/58, 75.86%), <i>chxA</i> (37/58, 63.79%), <i>hapA</i> (17/58, 29.31%), <i>ace</i> (22/58, 37.93%), and <i>cep</i> (20/58, 34.48%) etc. Such results show that 53.45% (31/58) of isolates harboured more than three virulence associated genes while <i>nanH</i>, <i>mshA</i>, <i>chxA</i>, <i>T6SS</i>, <i>T3SS</i>, <i>rtxA</i>, <i>hlyA</i>, <i>mshA</i>, <i>chxA</i>, <i>ace</i> and <i>cep</i> fingerprints were detected predominantly with corresponding ilea-loop test positive strains. ERIC-PCR also showed multiple target specific repetitive intergenic consensus sequence regions ranging from 2 to 8.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>This is an indication that the previously known non-pathogenic strains now harbour potential gastro-enteric virulence which may be controlled by more than one virulent gene dynamics. It also suggests a current potential shift in the virulence dynamics of <i>V</i>. <i>cholerae</i> strains recovered from the study area and a re-evaluated view of the previously non-pathogenic <i>V</i>. <i>cholerae</i> strains. Furthermore, the presence of such genes in <i>SA-NAG-Vc-1/139</i> strains indicates a potential public health related concern. Although these detected potential gastro-enteric associated genes may be implicated in sporadic gastroenteritis, our result has re-emphasized their probable public health concern as they may be involved in both endemic and severe gastroenteritis cases, which suggests the need for water routine monitoring or surveillance.</p>\\n </section>\\n </div>\",\"PeriodicalId\":100913,\"journal\":{\"name\":\"Medicine Advances\",\"volume\":\"2 4\",\"pages\":\"361-374\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-12-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/med4.88\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medicine Advances\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/med4.88\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medicine Advances","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/med4.88","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Emergence and detection of gastro-enteric virulence genes in non-pathogenic Vibrio cholerae: Implications for public health and water safety in African developmental regions
Background
Water, an indispensable component of life, has in recent times been associated with the distribution/spread of gastro-enteric diseases especially as safe water is a concern in Southern, Eastern and Western African developmental regions.
Methods
This study evaluated the molecular fingerprints of potential gastro-enteric associated virulence genes of 58 somatic antigen non-agglutinating Vibrio cholerae 1/139 (SA-NAG-Vc-1/139) strains from domestic water sources. Strains were isolated and characterized applying both culture-based-microbiological techniques and molecular-fingerprinting of target-specific identification genes using simplex/multiplex PCR assay and ERIC-PCR fingerprints.
Results
Our study revealed diverse gene-based indices vis T3SS (29/58, 50.00%), T6SS (33/58, 56.9%), rtxA (37/58, 63.79%), rtxC (12/58, 20.69%), NAG-stn/sto (13/58, 22.41%), prtV (17/58, 29.31%), hlyA (41/58, 70.69%), nanH (40/58, 68.97%), mshA (44/58, 75.86%), chxA (37/58, 63.79%), hapA (17/58, 29.31%), ace (22/58, 37.93%), and cep (20/58, 34.48%) etc. Such results show that 53.45% (31/58) of isolates harboured more than three virulence associated genes while nanH, mshA, chxA, T6SS, T3SS, rtxA, hlyA, mshA, chxA, ace and cep fingerprints were detected predominantly with corresponding ilea-loop test positive strains. ERIC-PCR also showed multiple target specific repetitive intergenic consensus sequence regions ranging from 2 to 8.
Conclusions
This is an indication that the previously known non-pathogenic strains now harbour potential gastro-enteric virulence which may be controlled by more than one virulent gene dynamics. It also suggests a current potential shift in the virulence dynamics of V. cholerae strains recovered from the study area and a re-evaluated view of the previously non-pathogenic V. cholerae strains. Furthermore, the presence of such genes in SA-NAG-Vc-1/139 strains indicates a potential public health related concern. Although these detected potential gastro-enteric associated genes may be implicated in sporadic gastroenteritis, our result has re-emphasized their probable public health concern as they may be involved in both endemic and severe gastroenteritis cases, which suggests the need for water routine monitoring or surveillance.