非致病性霍乱弧菌胃肠道毒力基因的出现和检测:对非洲发展中地区公共卫生和水安全的影响

Medicine Advances Pub Date : 2024-12-11 DOI:10.1002/med4.88
Bright Esegbuyota Igere
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引用次数: 0

摘要

水是生命不可缺少的组成部分,近年来与胃肠道疾病的分布/传播有关,特别是在南部、东部和西部非洲发展区域,安全用水是一个令人关切的问题。方法对来自生活水源的58株非凝集体抗原霍乱弧菌1/139 (SA-NAG-Vc-1/139)的潜在胃肠道相关毒力基因进行分子指纹图谱分析。采用培养微生物学技术和单/多重PCR及ERIC-PCR指纹图谱对目标特异性鉴定基因的分子指纹图谱进行分离和鉴定。结果研究结果显示,T3SS(29/58, 50.00%)、T6SS(33/58, 56.9%)、rtxA(37/58, 63.79%)、rtxC(12/58, 20.69%)、NAG-stn/sto(13/58, 22.41%)、prtV(17/58, 29.31%)、hlyA(41/58, 70.69%)、nanH(40/58, 68.97%)、mshA(44/58, 75.86%)、chxA(37/58, 63.79%)、hapA(17/58, 29.31%)、ace(22/58, 37.93%)、cep(20/58, 34.48%)等基因相关指数具有多样性。结果表明,53.45%(31/58)的菌株携带3个以上毒力相关基因,其中nanH、mshA、chxA、T6SS、T3SS、rtxA、hlyA、mshA、chxA、ace和cep指纹图谱以回肠环试验阳性菌株为主。ERIC-PCR还显示了多个靶向特异性重复基因间一致序列区域,范围为2 ~ 8个。结论这表明以前已知的非致病性菌株现在具有潜在的胃肠道毒力,这种毒力可能由多种毒力基因动力学控制。它还表明,从研究区域恢复的霍乱弧菌菌株的毒力动力学目前可能发生变化,并对以前非致病性霍乱弧菌菌株进行重新评估。此外,SA-NAG-Vc-1/139菌株中存在这些基因表明可能存在与公共卫生相关的问题。尽管这些检测到的潜在胃肠道相关基因可能与散发性胃肠炎有关,但我们的研究结果再次强调了它们可能引起的公共卫生问题,因为它们可能涉及地方性和严重胃肠炎病例,这表明需要对水进行常规监测或监测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Emergence and detection of gastro-enteric virulence genes in non-pathogenic Vibrio cholerae: Implications for public health and water safety in African developmental regions

Emergence and detection of gastro-enteric virulence genes in non-pathogenic Vibrio cholerae: Implications for public health and water safety in African developmental regions

Background

Water, an indispensable component of life, has in recent times been associated with the distribution/spread of gastro-enteric diseases especially as safe water is a concern in Southern, Eastern and Western African developmental regions.

Methods

This study evaluated the molecular fingerprints of potential gastro-enteric associated virulence genes of 58 somatic antigen non-agglutinating Vibrio cholerae 1/139 (SA-NAG-Vc-1/139) strains from domestic water sources. Strains were isolated and characterized applying both culture-based-microbiological techniques and molecular-fingerprinting of target-specific identification genes using simplex/multiplex PCR assay and ERIC-PCR fingerprints.

Results

Our study revealed diverse gene-based indices vis T3SS (29/58, 50.00%), T6SS (33/58, 56.9%), rtxA (37/58, 63.79%), rtxC (12/58, 20.69%), NAG-stn/sto (13/58, 22.41%), prtV (17/58, 29.31%), hlyA (41/58, 70.69%), nanH (40/58, 68.97%), mshA (44/58, 75.86%), chxA (37/58, 63.79%), hapA (17/58, 29.31%), ace (22/58, 37.93%), and cep (20/58, 34.48%) etc. Such results show that 53.45% (31/58) of isolates harboured more than three virulence associated genes while nanH, mshA, chxA, T6SS, T3SS, rtxA, hlyA, mshA, chxA, ace and cep fingerprints were detected predominantly with corresponding ilea-loop test positive strains. ERIC-PCR also showed multiple target specific repetitive intergenic consensus sequence regions ranging from 2 to 8.

Conclusions

This is an indication that the previously known non-pathogenic strains now harbour potential gastro-enteric virulence which may be controlled by more than one virulent gene dynamics. It also suggests a current potential shift in the virulence dynamics of V. cholerae strains recovered from the study area and a re-evaluated view of the previously non-pathogenic V. cholerae strains. Furthermore, the presence of such genes in SA-NAG-Vc-1/139 strains indicates a potential public health related concern. Although these detected potential gastro-enteric associated genes may be implicated in sporadic gastroenteritis, our result has re-emphasized their probable public health concern as they may be involved in both endemic and severe gastroenteritis cases, which suggests the need for water routine monitoring or surveillance.

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