Serkan Erdemir, Duygu Aydin, Mehmet Oguz, Ozcan Kocyigit, Sait Malkondu
{"title":"构建生态友好、生物兼容、比例计量的近红外荧光传感器,用于快速检测真实样品和活细胞中的痕量Pd2+","authors":"Serkan Erdemir, Duygu Aydin, Mehmet Oguz, Ozcan Kocyigit, Sait Malkondu","doi":"10.1016/j.jhazmat.2025.137463","DOIUrl":null,"url":null,"abstract":"The increasing industrial use of palladium has led to its environmental accumulation, raising concerns about its toxicity to aquatic life and human health. Therefore, fluorescent probes capable of detecting Pd<sup>2+</sup> are highly beneficial. With this objective, a new near-infrared (NIR) fluorescent probe based on a cyanine dye, 2 ((<em>E</em>)-2-((<em>E</em>)-2-((dimethylcarbamothioyl)oxy)-3-(2-((<em>Z</em>)-1,3,3-trimethylindolin-2ylidene)ethylidene)cyclohex −1-en-1-yl)vinyl)-1,3,3-trimethyl-3H-indol-1-ium iodide (CNS), was synthesized for selective and rapid detection of Pd<sup>2+</sup>. The detection reaction followed the elimination of thiocarbamate moiety, leading to the highly fluorescent product. CNS demonstrated remarkable sensitivity (detection limit: 0.105 μM), high selectivity, short response time (1.0 min), long lifetime (0.88 ns), and easily detectable color changes upon Pd<sup>2+</sup> exposure. A CNS-loaded TLC strip integrated with a smartphone detection system was able to detect Pd<sup>2+</sup> in solutions, soil, and drug samples. In addition, CNS enabled concentration-dependent detection of Pd<sup>2+</sup> in onion roots and epidermis. Because of low cytotoxicity, good membrane permeability, NIR fluorescence, and high contrast, CNS has been successfully applied to Pd<sup>2+</sup> bioimaging in living cells, targeting mitochondria. Compared to existing probes, CNS offers superior sensitivity, selectivity, and adaptability for sensing applications.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"20 1","pages":""},"PeriodicalIF":11.3000,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Building an eco-friendly, biocompatible, and ratiometric NIR fluorescent sensor for the rapid detection of trace Pd2+ in real samples and living cells\",\"authors\":\"Serkan Erdemir, Duygu Aydin, Mehmet Oguz, Ozcan Kocyigit, Sait Malkondu\",\"doi\":\"10.1016/j.jhazmat.2025.137463\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The increasing industrial use of palladium has led to its environmental accumulation, raising concerns about its toxicity to aquatic life and human health. Therefore, fluorescent probes capable of detecting Pd<sup>2+</sup> are highly beneficial. With this objective, a new near-infrared (NIR) fluorescent probe based on a cyanine dye, 2 ((<em>E</em>)-2-((<em>E</em>)-2-((dimethylcarbamothioyl)oxy)-3-(2-((<em>Z</em>)-1,3,3-trimethylindolin-2ylidene)ethylidene)cyclohex −1-en-1-yl)vinyl)-1,3,3-trimethyl-3H-indol-1-ium iodide (CNS), was synthesized for selective and rapid detection of Pd<sup>2+</sup>. The detection reaction followed the elimination of thiocarbamate moiety, leading to the highly fluorescent product. CNS demonstrated remarkable sensitivity (detection limit: 0.105 μM), high selectivity, short response time (1.0 min), long lifetime (0.88 ns), and easily detectable color changes upon Pd<sup>2+</sup> exposure. A CNS-loaded TLC strip integrated with a smartphone detection system was able to detect Pd<sup>2+</sup> in solutions, soil, and drug samples. In addition, CNS enabled concentration-dependent detection of Pd<sup>2+</sup> in onion roots and epidermis. Because of low cytotoxicity, good membrane permeability, NIR fluorescence, and high contrast, CNS has been successfully applied to Pd<sup>2+</sup> bioimaging in living cells, targeting mitochondria. Compared to existing probes, CNS offers superior sensitivity, selectivity, and adaptability for sensing applications.\",\"PeriodicalId\":361,\"journal\":{\"name\":\"Journal of Hazardous Materials\",\"volume\":\"20 1\",\"pages\":\"\"},\"PeriodicalIF\":11.3000,\"publicationDate\":\"2025-02-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Hazardous Materials\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jhazmat.2025.137463\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hazardous Materials","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.jhazmat.2025.137463","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Building an eco-friendly, biocompatible, and ratiometric NIR fluorescent sensor for the rapid detection of trace Pd2+ in real samples and living cells
The increasing industrial use of palladium has led to its environmental accumulation, raising concerns about its toxicity to aquatic life and human health. Therefore, fluorescent probes capable of detecting Pd2+ are highly beneficial. With this objective, a new near-infrared (NIR) fluorescent probe based on a cyanine dye, 2 ((E)-2-((E)-2-((dimethylcarbamothioyl)oxy)-3-(2-((Z)-1,3,3-trimethylindolin-2ylidene)ethylidene)cyclohex −1-en-1-yl)vinyl)-1,3,3-trimethyl-3H-indol-1-ium iodide (CNS), was synthesized for selective and rapid detection of Pd2+. The detection reaction followed the elimination of thiocarbamate moiety, leading to the highly fluorescent product. CNS demonstrated remarkable sensitivity (detection limit: 0.105 μM), high selectivity, short response time (1.0 min), long lifetime (0.88 ns), and easily detectable color changes upon Pd2+ exposure. A CNS-loaded TLC strip integrated with a smartphone detection system was able to detect Pd2+ in solutions, soil, and drug samples. In addition, CNS enabled concentration-dependent detection of Pd2+ in onion roots and epidermis. Because of low cytotoxicity, good membrane permeability, NIR fluorescence, and high contrast, CNS has been successfully applied to Pd2+ bioimaging in living cells, targeting mitochondria. Compared to existing probes, CNS offers superior sensitivity, selectivity, and adaptability for sensing applications.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.