IF 5.1 1区 农林科学 Q1 SOIL SCIENCE
Jie Zhou, Sebastian Loeppmann, Haishui Yang, Matthias Gube, Lingling Shi, Johanna Pausch, Michaela A. Dippold
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引用次数: 0

摘要

人们对根源碳(C)进入土壤微生物群落的途径知之甚少,而根源碳进入土壤微生物群落的途径是对丛枝菌根真菌(AMF)和氮(N)肥的反应。番茄的菌根缺陷突变体(菌根定植减少:rmc)及其菌根野生型祖先(MYC)被用来控制 AMF 的形成。通过对中性(NLFAs)和磷脂脂肪酸(PLFAs)的 13C 图谱进行连续 16 周的 13CO2 标记,以量化活性微生物的光合 C 分配。真菌生物标志物(PLFA 16:1ω5c、NLFA 16:1ω5c、PLFA 18:2ω6,9的总和)中的13C掺入量在16周内随着时间的推移而增加,全部同化C的4.62%掺入了AMF。与菌体生物量(PLFA 16:1ω5c,0.12-0.25%)相比,更多的 13C 被分配到 AMF 储存化合物(NLFA 16:1ω5c,3.1-4.1%)中。此外,AMF共生改变了微生物群落的组成,导致细菌和食腐真菌的 13C 含量低于 rmc 植物。这表明细菌和噬菌真菌对根源 C 的利用率较低,但更倾向于将较老的 C 化合物作为能量来源。然而,施氮肥会降低AMF的丰度,因此与未施肥土壤相比,根源C在PLFA和NLFA 16:1ω5c中的结合率较低,这是由于地上生物量中固定的C增加,导致C分配减少。我们的研究结果表明,根系来源的碳可以通过贮存在其生殖器官中而被AMF固碳,但AMF优先分配碳可能会牺牲流向其他微生物群的碳。总之,我们的研究结果证实,菌根植物对细菌和食腐真菌的碳吸收有更大的影响,但这在很大程度上取决于氮肥。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Linking microbial community dynamics to rhizosphere carbon flow depend on arbuscular mycorrhizae and nitrogen fertilization

Little is known about the path of root-derived carbon (C) into soil microbial communities in response to arbuscular mycorrhizal fungi (AMF) and nitrogen (N) fertilization. A mycorrhiza defective mutant of tomato (reduced mycorrhizal colonization: rmc) and its mycorrhizal wild type progenitor (MYC) were used to control for the formation of AMF. 16-week continuous 13CO2 labeling was performed to quantify the photosynthetic C allocation in active microorganisms via 13C profiles of neutral (NLFAs) and phospholipid fatty acids (PLFAs). The 13C incorporation into fungal biomarker (the sum of PLFA 16:1ω5c, NLFA 16:1ω5c, PLFA 18:2ω6,9) increased with time over 16 weeks, and 4.62% of totally assimilated C was incorporated into AMF. More 13C was allocated into AMF storage compounds (NLFA 16:1ω5c, 3.1–4.1%) than hyphal biomass (PLFA 16:1ω5c, 0.12–0.25%). Furthermore, AMF symbiosis shifted microbial community composition, resulting in a lower 13C incorporation into bacteria and saprotrophic fungi compared to rmc plants. This suggests a lower use of root-derived C by bacteria and saprotrophic fungi but preference to older C compounds as energy sources. However, N fertilization decreased AMF abundance and subsequently less root-derived C was incorporated into PLFA and NLFA 16:1ω5c in relative to unfertilized soils, due to less C allocation caused by an increased C immobilization in the aboveground biomass. Our findings suggested that root-derived C can be sequestered by AMF through storage in their reproductive organs, but the preferential C allocation to AMF might be at the expense of C flow to other microbial groups. Overall, our results confirmed that mycorrhizal plants exert a greater influence on C incorporation into bacteria and saprotrophic fungi, which, however, is highly dependent on N fertilization.

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来源期刊
Biology and Fertility of Soils
Biology and Fertility of Soils 农林科学-土壤科学
CiteScore
11.80
自引率
10.80%
发文量
62
审稿时长
2.2 months
期刊介绍: Biology and Fertility of Soils publishes in English original papers, reviews and short communications on all fundamental and applied aspects of biology – microflora and microfauna - and fertility of soils. It offers a forum for research aimed at broadening the understanding of biological functions, processes and interactions in soils, particularly concerning the increasing demands of agriculture, deforestation and industrialization. The journal includes articles on techniques and methods that evaluate processes, biogeochemical interactions and ecological stresses, and sometimes presents special issues on relevant topics.
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