杏仁核阿片系统在应激对雄性Wistar大鼠学习后睡眠模式的影响中的作用。

IF 2.7 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Mehdi Graily-Afra, Farideh Bahrami, Zahra Bahari, Hedayat Sahraei, Zeinab Shankayi, Ali Gharib
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引用次数: 0

摘要

目的:三个生理过程相互作用:睡眠、学习和压力。了解压力如何影响睡眠、学习和记忆之间的联系并与之相互作用是很重要的,因为人们早就认识到睡眠在记忆巩固和学习中起着至关重要的作用。本研究旨在通过在基底外侧杏仁核(BLA)注射纳洛酮,揭示应激、学习和睡眠之间的相互作用,以及阿片系统的功能及其对海马脑源性神经营养因子(BDNF)产生的影响。材料与方法:11组雄性Wistar大鼠(n=77)分别植入脑电图(EEG)和肌电(EMG)记录电极,双侧BLA区插管。在实施静止压力方案前3小时和实施后3小时,记录受试者的快速眼动(REM)和非快速眼动(NREM)睡眠和清醒步骤,并连续3天使用Barnes迷宫学习。同时,48小时后品尝动物的记忆。在应激学习程序开始前,连续3次向每个BLA注射纳洛酮,剂量为0.05 μg或0.1 μg,体积为0.5 μl。学习和压力的分子生物标志物BDNF也被检测。结果:研究表明,静止应激模型降低了快速眼动期和非快速眼动期睡眠。另一方面,将学习技巧付诸实践会导致更多的快速眼动和非快速眼动睡眠,并且学习后的压力情况不会阻止这种上升。在BLA区域注射纳洛酮也能增强学习和记忆,防止与压力相关的快速眼动和非快速眼动睡眠缺失。此外,应激降低海马区的BDNF表达。在整个学习过程中,海马中BDNF表达升高,在BLA区给予纳洛酮也提高了海马中BDNF表达。结论:应激可降低海马区快速眼动期、非快速眼动期及BDNF表达。在压力下,使用学习协议会增加快速眼动睡眠、非快速眼动睡眠和BDNF。注射纳洛酮可改善脑梗死患者的记忆和学习能力,减少应激性记忆丧失。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of the amygdala opioid system in the effects of stress on the post-learning sleep patterns of male Wistar rats.

Objectives: Three physiological processes interact: sleep, learning, and stress. It is essential to understand how stress affects and interacts with the link between sleep, learning, and memory since it has long been recognized that sleep plays a crucial role in memory consolidation and learning. Through naloxone injection in the baso lateral amygdala (BLA), this study intends to shed light on the interactions between stress, learning, and sleep, as well as the function of the opioid system and its impact on brain-derived neurotrophic factor (BDNF) production in the hippocampus.

Materials and methods: Male Wistar rats (n=77) in eleven groups were implanted with electroencephalogram (EEG) and electromyography (EMG) recording electrodes, and the BLA area was bilaterally cannulated. Recordings of Rapid Eye Movement (REM) and Non-Rapid Eye Movement (NREM) sleep and wakefulness steps were made for the three hours prior to and three hours following the implementation of the immobility stress protocol and learning with the Barnes maze for three consecutive days. Also, the animals' memory was tasted 48 hr later. Before the stress and learning procedure, naloxone was injected into each BLA three times in a row at a dosage of 0.05 μg or 0.1 μg in a volume of 0.5 μl. A molecular biomarker of learning and stress, BDNF, was also examined.

Results: The study demonstrated that the immobility stress model lowers REM and NREM sleep. On the other hand, putting the learning technique into practice results in more REM and NREM sleep, and stress situations do not stop this rise after learning. Naloxone injections in the BLA region also enhance learning and memory, preventing stress-related REM and NREM sleep loss. Additionally, stress lowers BDNF expression in the hippocampal region. BDNF expression rises in the hippocampus throughout the learning process, and naloxone administration in the BLA area also raises BDNF expression in the hippocampus.

Conclusion: Stress generally reduces REM, NREM, and BDNF expression in the hippocampal region. Under stress, using the learning protocol increases REM, NREM sleep, and BDNF. Naloxone injection in BLA improves memory and learning, reducing stress-induced memory loss.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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