Laisa Kindely Ramos de Oliveira, Conrado Dias do Nascimento Neto, Amy Brian Costa E Silva, Stela Maris Wanderley Rocha, Patrícia Roccon Bianchi, André Gustavo de Sousa Galdino, Daniela Nascimento Silva
{"title":"Physicochemical characterization and effects of monetite obtained from titania-reinforced eggshell on bone repair: a new possibility for tissue bioengineering?","authors":"Laisa Kindely Ramos de Oliveira, Conrado Dias do Nascimento Neto, Amy Brian Costa E Silva, Stela Maris Wanderley Rocha, Patrícia Roccon Bianchi, André Gustavo de Sousa Galdino, Daniela Nascimento Silva","doi":"10.1007/s00784-025-06195-7","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To carry out physicomechanical characterization of the HA/DCPA/TiO<sub>2</sub> and to evaluate the tissue repair in rat calvaria.</p><p><strong>Methods: </strong>Two bone defects were made in the calvaria of 36 Wistar rats, divided into groups: HA/DCPA, HA/DCPA/TiO<sub>2</sub> and sham (blood clot). The animals were euthanized at 30, 60 and 90 days and calvaria slides were processed with hematoxylin/eosin. The newly formed bone, connective tissue, biomaterial remnant, and total tissue repair percentages were calculated in relation to the total defect area. The HA/DCPA/TiO<sub>2</sub> was characterized structurally by scanning electron microscopy (SEM), and chemically by energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). It was submitted to apparent density (AD), apparent porosity (AP), water absorption (WA) and compressive strength (CS) physical tests. The ANOVA test was applied, followed by Turkey's test and Student's t-test (p ≤ 0,05).</p><p><strong>Results: </strong>The SEM showed biomaterials inside the bone defects and newly formed bone. EDS identified oxygen, calcium, phosphorus, and titanium in the sample. The HA/DCPA/TiO<sub>2</sub> and HA/DCPA groups presented a total tissue repair area that was larger than the sham group (p < 0.001).</p><p><strong>Conclusions: </strong>The physical-mechanical assays showed that HA/DCPA/TiO<sub>2</sub> has AD and CS properties within the limits of trabecular bone and with values higher than HA/DCPA.HA/DCPA/TiO<sub>2</sub> presented higher densification and compressive strength rates than HA/DCPA.</p><p><strong>Clinical relevance: </strong>Potential as a scaffold for bone.</p>","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 2","pages":"108"},"PeriodicalIF":3.1000,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Oral Investigations","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00784-025-06195-7","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Physicochemical characterization and effects of monetite obtained from titania-reinforced eggshell on bone repair: a new possibility for tissue bioengineering?
Objectives: To carry out physicomechanical characterization of the HA/DCPA/TiO2 and to evaluate the tissue repair in rat calvaria.
Methods: Two bone defects were made in the calvaria of 36 Wistar rats, divided into groups: HA/DCPA, HA/DCPA/TiO2 and sham (blood clot). The animals were euthanized at 30, 60 and 90 days and calvaria slides were processed with hematoxylin/eosin. The newly formed bone, connective tissue, biomaterial remnant, and total tissue repair percentages were calculated in relation to the total defect area. The HA/DCPA/TiO2 was characterized structurally by scanning electron microscopy (SEM), and chemically by energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). It was submitted to apparent density (AD), apparent porosity (AP), water absorption (WA) and compressive strength (CS) physical tests. The ANOVA test was applied, followed by Turkey's test and Student's t-test (p ≤ 0,05).
Results: The SEM showed biomaterials inside the bone defects and newly formed bone. EDS identified oxygen, calcium, phosphorus, and titanium in the sample. The HA/DCPA/TiO2 and HA/DCPA groups presented a total tissue repair area that was larger than the sham group (p < 0.001).
Conclusions: The physical-mechanical assays showed that HA/DCPA/TiO2 has AD and CS properties within the limits of trabecular bone and with values higher than HA/DCPA.HA/DCPA/TiO2 presented higher densification and compressive strength rates than HA/DCPA.
Clinical relevance: Potential as a scaffold for bone.
期刊介绍：
The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.
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