荆芥叶提取物抗糖尿病活性的体内实验研究及组织病理学评价

Q3 Pharmacology, Toxicology and Pharmaceutics

Phytomedicine Plus Pub Date : 2025-02-01 DOI:10.1016/j.phyplu.2025.100734

Sunayana Mali , Gaurav Jain , Jyotiram Sawale , Audumbar Mali

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引用次数: 0

摘要

本研究研究了马哈拉施特拉邦索拉普尔地区新发现的新植物C. solapurense （Crinum solapurense）的生物活性，解决了其特性的文献空白。在这项调查中，我们进行了植物的植物化学，粉末特性和体内抗糖尿病活性。利用地塞米松诱导的糖尿病大鼠体内实验，对茄水仙叶乙醇提取物的生物活性潜力和组织病理学研究提供了有价值的见解。目的评价荆芥叶提取物对雄性白化Wistar大鼠体内模型的药理作用和组织病理学研究。方法连续提取乙醇提取物并进行植物化学分析。观察地塞米松诱导的糖尿病大鼠体内抗糖尿病活性及组织病理学变化。结果地塞米松诱导大鼠糖尿病后，各组大鼠分别用标准降糖药格列本脲（GLC）蒸馏水和低、中、高剂量的金楝叶提取物（100.00 mg/kg、200.00 mg/kg、200.00 mg/kg）处理；400.00 mg/kg。山楂叶提取物可显著降低地塞米松诱导的雄性白化Wistar大鼠的血糖水平。同时，以较高剂量（400 mg/kg体重）的水芹叶提取物效果最好。进水量&；地塞米松诱导的雄性白化Wistar大鼠的食物摄取量显著减少，而低、中、高剂量的金盏花叶提取物对大鼠的体重减轻作用最小。在组织病理学研究中，较高剂量（400.00 mg/kg）的茄水芹对地塞米松诱导的糖尿病大鼠苏木精和伊红染色胰腺细胞的朗格汉斯β胰岛细胞再生有非常显著的影响。结论新发现的山楂叶乙醇提取物对地塞米松诱导的雄性白化Wistar大鼠具有体内抗糖尿病作用，可预防糖尿病的多种并发症。它可能对糖尿病（DM）的治疗有用。

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Investigation of Crinum solapurense leaves extract for antidiabetic activity and histopathological evaluation in an in-vivo experimental model

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Investigation of Crinum solapurense leaves extract for antidiabetic activity and histopathological evaluation in an in-vivo experimental model

Background

This study investigates the biological activity of the newly discovered novel C. solapurense (Crinum solapurense) plant from the Solapur, Maharashtra region, addressing a literature gap on its properties. In this investigation, we have performed the plant's phytochemical, powder characteristics, and in-vivo antidiabetic activity. Evaluating ethanolic extract of C. solapurense leaves for in-vivo study gives valuable insights into its bioactive potential and histopathological investigation in dexamethasone-induced diabetic rats.

Purpose

To evaluate the pharmacological activity and histopathological investigation of C. solapurense leaf extracts in an in-vivo experimental model in male albino Wistar rats.

Methods

Ethanolic leaf extracts underwent successive extraction and phytochemical profiling. In-vivo antidiabetic activity and histopathological investigation were assessed in dexamethasone-induced diabetic rats.

Results

After inducing diabetes in rats by using dexamethasone, various groups of rats were treated with distilled water, a standard antidiabetic drug used as a Glibenclamide (GLC), and lower, medium, and higher doses of C. solapurense leaf extracts like 100.00 mg/kg, 200.00 mg/kg & 400.00 mg/kg respectively. The C. solapurense leaf extracts significantly reduced blood glucose levels (BGL) in dexamethasone-induced male albino Wistar rats. At the same time, the best results were obtained at higher doses of C. solapurense leaf extracts (400 mg/kg body weight). The water intake & food intake in dexamethasone-induced male albino Wistar rats were significantly decreased however weight loss of rats was minimized at lower, medium, and higher doses of C. solapurense leaves extract. In the histopathological study, the higher dose of C. solapurense (400.00 mg/kg) showed very significant effects for the regeneration of cells for beta islets of Langerhans on hematoxylin and eosin-stained pancreas cells in dexamethasone-induced diabetic rats.

Conclusion

The ethanolic leaf extract of newly discovered C. solapurense possesses in-vivo antidiabetic properties in dexamethasone-induced male albino Wistar rats, preventing different complications of diabetes mellitus. It could be useful for the treatment of diabetes mellitus (DM).

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