烟酰胺核苷假尿苷二磷酸的酶合成与表征

IF 1.5 4区化学 Q3 CHEMISTRY, ORGANIC

Tetrahedron Letters Pub Date : 2025-01-30 DOI:10.1016/j.tetlet.2024.155432

Yinghan Hu , Xueying Wang , Yanzhe Huang , Xiaojia Guo , Haizhao Xue , Martin Pfeiffer , Bernd Nidetzky , Zongbao K. Zhao

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引用次数: 0

摘要

烟酰胺腺嘌呤二核苷酸（NAD）类似物在化学和生物学上有着广泛的应用。本研究利用烟酸单核苷酸腺苷转移酶突变体催化三磷酸假尿嘧啶和烟酰胺单核苷酸偶联，合成了一种新的NAD类似物——烟酰胺核苷伪尿嘧啶二磷酸（NpUD）。研究发现，NpUD对大肠杆菌中常见的一种焦磷酸酶NudC比NAD更稳定。有趣的是，NpUD可以作为几种氧化还原酶的氧化还原辅助因子，其中突变体甲醇脱氢酶的NpUD活性比NAD高1.9倍。总的来说，研究结果为探索NpUD在其他应用中的应用提供了机会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Enzymatic synthesis and characterization of nicotinamide riboside pseudouridine diphosphate

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Enzymatic synthesis and characterization of nicotinamide riboside pseudouridine diphosphate

Nicotinamide adenine dinucleotide (NAD) analogs are of great interests for chemical and biological applications. In this work, a novel NAD analog, nicotinamide riboside pseudouridine diphosphate (NpUD) was synthesized via coupling of pseudouridine triphosphate and nicotinamide mononucleotide catalyzed by a mutant of nicotinic acid mononucleotide adenylyltransferase. It was found that NpUD is more stable than NAD against a common pyrophosphatase NudC found in Escherichia coli. Interestingly, NpUD could function as redox cofactor for several oxidoreductases, among them a mutant methanol dehydrogenase showed 1.9-fold higher activity with NpUD than that with NAD. Overall, the results offer opportunities to explore NpUD in other applications.

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来源期刊

Tetrahedron Letters 化学-有机化学

CiteScore

3.50

自引率

5.60%

发文量

521

审稿时长

28 days

期刊介绍： Tetrahedron Letters provides maximum dissemination of outstanding developments in organic chemistry. The journal is published weekly and covers developments in techniques, structures, methods and conclusions in experimental and theoretical organic chemistry. Rapid publication of timely and significant research results enables researchers from all over the world to transmit quickly their new contributions to large, international audiences.