三花草（Desmodium triflorum）体外中和胃酸协同作用的研究直流。和蒲公英。在抗酸片制剂中

IF 1.9 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2025-02-01 DOI:10.1016/j.hermed.2024.100981

Bhavantha Dias , Banukie Jayasuriya , Dilanthi Herath , Menuka Arawwawala

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引用次数: 0

摘要

本研究旨在评价两种陆生药用植物的体外联合抗酸活性；三花菊和广藿香，并随后使用其提取物配制抗酸剂型。方法采用不同的体外实验方法，对三花草全株和海参叶粗水提物组合对蒸馏水的中和胃酸活性进行评价。然后，以3:1的浓度组合对三花草和海氏三花草进行膜透析，并在瓦提尔人工胃模型中测定所得馏分。采用高效液相色谱（HPLC）和傅里叶变换红外（FTIR）对同一提取物组合进行表征，并将其用于中药抗酸片的配制。结果以3:1的组合比（三花草：海棠）具有显著的抑菌活性(P <；酸中和效果试验（4.94±0.00结束pH）和酸中和持续时间试验（403.4±4.6 秒）0.05)。组合指数分析表明，3:1组合进一步产生协同抗酸活性。膜透析同一提取物的高分子和低分子组分显示出彼此相当的生物活性(P >；抗酸持续时间测定（分别为182.78±3.66 秒和218.18±2.34 秒）。使用测试植物的3:1组合配制的草药片剂显示出药学上可接受的物理特性以及显着的酸中和(P <；0.05)。结论三花草与海棠提取物以3:1的浓度组合具有协同中和胃酸的作用，可能与不同分子量的植物化学物质有关。该组合物可进一步用于具有足够体外活性的抗酸片制剂中。

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Exploiting in vitro gastric acid-neutralising synergy between Desmodium triflorum (L.) DC. and Pogostemon heyneanus Benth. in an antacid tablet formulation

Introduction

This study aimed to evaluate the combinative in vitro antacid activity of two terrestrial medicinal plants; Desmodium triflorum and Pogostemon heyneanus, and to subsequently formulate an antacid dosage form using their extracts.

Methods

The gastric acid-neutralising activity of crude aqueous extract combinations of D. triflorum whole plant and P. heyneanus leaves was evaluated using different in vitro assays, against distilled water. Then, the 3:1 concentration combination of D. triflorum and P. heyneanus was membrane dialysed and the resulting fractions were assayed in Vatier’s artificial stomach model. The same extract combination was characterised by high-performance liquid chromatography (HPLC) and fourier transform infrared (FTIR) analyses and then used in the formulation of herbal antacid tablets.

Results

The combination ratio of 3:1 (D. triflorum: P. heyneanus) demonstrated a significant activity (P < 0.05) in acid-neutralising effect assay (4.94 ± 0.00 end pH) and acid neutralisation duration assay (403.4 ± 4.6 seconds). The 3:1 combination further resulted in synergistic antacid activity, evidenced by the combinative index analysis. Membrane dialysed high- and low-molecular fractions of the same extract demonstrated bioactivities comparable to each other (P > 0.05) in antacid duration assay (182.78 ± 3.66 seconds and 218.18 ± 2.34 seconds respectively). The herbal tablet formulated using the 3:1 combination of the test plants showed pharmaceutically acceptable physical characteristics along with a significant acid-neutralisation (P < 0.05).

Conclusions

The 3:1 concentration combination of D. triflorum and P. heyneanus extracts resulted in synergistic gastric acid-neutralising effects, possibly with the aid of phytochemicals with various molecular weights. The combination can be further used in an antacid tablet formulation with sufficient in vitro activity.

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.