沙特阿拉伯迭戈血型的遗传背景

IF 3.6 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Journal of King Saud University - Science Pub Date : 2024-12-01 DOI:10.1016/j.jksus.2024.103571

Amr J. Halawani , Saif Elden B. Abdalla , Abdullah Meshi , Ghalia Shamlan , Mahmoud M. Habibullah

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引用次数: 0

摘要

背景：多次输血患者在沙特阿拉伯吉赞地区很普遍。同种异体免疫可能发生在红细胞不相容的情况下。因此，对其他血型进行广泛的血清分型是必不可少的。Diego （DI）血型系统的两个等位基因DI*A和DI*B分别编码DI主要抗原DI （A + B -）和DI （A - B +）。Anti-Dia和anti-Dib可能涉及输血反应和妊娠问题。本研究旨在调查沙特阿拉伯吉赞省DI血型的等位基因和基因型频率。方法采集吉赞省沙特献血者血样150份。提取DNA，设计序列特异性引物扩增区分DI*A和DI*B等位基因的SNV区（rs2285644）。对所得PCR扩增子进行测序。结果DI*B等位基因频率为100%，未见DI*A等位基因。因此，检测到的唯一基因型为DI*B/DI*B，阳性率为100%。结论本研究报道了沙特阿拉伯DI血型系统的等位基因频率和基因型频率。本研究可能有助于在吉赞地区建立国家血型数据库。此外，它可以通过提供匹配的血液单位来帮助降低同种免疫的风险。

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Genetic background of Diego blood group in Saudi Arabia

Background

Multiply-transfused patients are prevalent in Jazan region, Saudi Arabia. Alloimmunization may occur in case of red cell incompatibility. Therefore, extensive serotyping for other blood groups is essential. Two alleles of the Diego (DI) blood group system, DI*A and DI*B encode the main DI antigens Di(a + b–) and Di(a–b+), respectively. Anti-Di^a and anti-Di^b can be involved in transfusion reactions and pregnancy issues. This study aimed to investigate the allele and genotype frequencies of the DI blood groups in Jazan Province of Saudi Arabia.

Methods

One-hundred-fifty samples were collected from Saudi blood donors in Jazan Province. DNA was extracted and sequence-specific primers were designed to amplify the SNV region (rs2285644), which distinguishes the DI*A allele from the DI*B allele. The resulting PCR amplicons were sequenced.

Results

The frequency of DI*B alleles was 100 %, while the DI*A allele was not observed. Therefore, the only detected genotype was DI*B/DI*B at 100 %.

Conclusions

This study reported the allele and genotype frequencies of the DI blood group system in Saudi Arabia. This study may help establish a national database for blood groups in Jazan region. Moreover, it may help to reduce the risk of alloimmunization by providing matching blood units.

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来源期刊

Journal of King Saud University - Science Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

2.60%

发文量

642

审稿时长

49 days

期刊介绍： Journal of King Saud University – Science is an official refereed publication of King Saud University and the publishing services is provided by Elsevier. It publishes peer-reviewed research articles in the fields of physics, astronomy, mathematics, statistics, chemistry, biochemistry, earth sciences, life and environmental sciences on the basis of scientific originality and interdisciplinary interest. It is devoted primarily to research papers but short communications, reviews and book reviews are also included. The editorial board and associated editors, composed of prominent scientists from around the world, are representative of the disciplines covered by the journal.