Doaa M. El-Wakil , Aya M. Nasr , Alaa R. Awad , Iman H. Bassyouni , Nadia M.H. Madany
{"title":"类风湿关节炎患者外周血单个核细胞中ciRS-7和miR-7的表达规律","authors":"Doaa M. El-Wakil , Aya M. Nasr , Alaa R. Awad , Iman H. Bassyouni , Nadia M.H. Madany","doi":"10.1016/j.ejr.2024.12.006","DOIUrl":null,"url":null,"abstract":"<div><div>Background: Non-coding RNAs (ncRNAs), including miR-7 and ciRS-7, are implicated in many autoimmune diseases, but their roles in rheumatoid arthritis (RA) stay largely unexplored. <em>Aim of the work:</em> To assess miR-7 and ciRS-7 as diagnostic biomarkers for RA by comparing their expression patterns in patients <em>vs.</em> controls and correlating their profiles with anti-cyclic citrullinated peptide (anti-CCP) levels and clinical findings. <em>Patients and methods:</em> Forty-five patients and 45 controls were enrolled. Enzyme-linked immunosorbent assay (ELISA) was performed to assess plasma anti-CCP levels, and reverse transcription polymerase chain reaction (RT-PCR) was conducted to determine miR-7 and ciRS-7 expression levels. <em>Results:</em> All cases were female with a mean age of 39.6 ± 10.2 years. Cases exhibited significantly lower levels of miR-7 expression than control (median, 0.51 <em>vs.</em> 1.03; <em>p</em> < 0.001), while a significant elevation in ciRS-7 was observed in cases (median, 4.29 <em>vs.</em> 0.93; <em>p</em> < 0.001). Neither miR-7 nor ciRS-7 showed a significant correlation with anti-CCP levels. Meanwhile, miR-7 and ciRS-7 levels significantly correlated with the duration of RA (<em>r<sub>s</sub></em> = 0.32, <em>p</em> = 0.03; <em>r<sub>s</sub></em> = 0.4, <em>p</em> = 0.01, respectively), while the anti-CCP levels demonstrated a significant negative correlation with disease duration (<em>r<sub>s</sub></em>= −0.33, <em>p</em> = 0.03). miR-7 and ciRS-7 significantly distinguish cases from controls at cut-off values of < 0.81 and > 1.7, respectively (AUC = 0.98 and 0.99; <em>p</em> < 0.001), and their combination achieved 100 % diagnostic accuracy, with an AUC of 1 (<em>p</em> < 0.001). <em>Conclusions:</em> miR-7 and ciRS-7 have an inverse relationship, suggesting that ciRS-7 could modulate miR-7 regulatory functions. Both miR-7 and ciRS-7 could be used as diagnostic biomarkers for RA, and their combination could improve RA prediction.</div></div>","PeriodicalId":46152,"journal":{"name":"Egyptian Rheumatologist","volume":"47 2","pages":"Pages 80-84"},"PeriodicalIF":1.0000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Expression patterns of ciRS-7 and miR-7 in peripheral blood mononuclear cells of rheumatoid arthritis patients\",\"authors\":\"Doaa M. El-Wakil , Aya M. Nasr , Alaa R. Awad , Iman H. Bassyouni , Nadia M.H. Madany\",\"doi\":\"10.1016/j.ejr.2024.12.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Background: Non-coding RNAs (ncRNAs), including miR-7 and ciRS-7, are implicated in many autoimmune diseases, but their roles in rheumatoid arthritis (RA) stay largely unexplored. <em>Aim of the work:</em> To assess miR-7 and ciRS-7 as diagnostic biomarkers for RA by comparing their expression patterns in patients <em>vs.</em> controls and correlating their profiles with anti-cyclic citrullinated peptide (anti-CCP) levels and clinical findings. <em>Patients and methods:</em> Forty-five patients and 45 controls were enrolled. Enzyme-linked immunosorbent assay (ELISA) was performed to assess plasma anti-CCP levels, and reverse transcription polymerase chain reaction (RT-PCR) was conducted to determine miR-7 and ciRS-7 expression levels. <em>Results:</em> All cases were female with a mean age of 39.6 ± 10.2 years. Cases exhibited significantly lower levels of miR-7 expression than control (median, 0.51 <em>vs.</em> 1.03; <em>p</em> < 0.001), while a significant elevation in ciRS-7 was observed in cases (median, 4.29 <em>vs.</em> 0.93; <em>p</em> < 0.001). Neither miR-7 nor ciRS-7 showed a significant correlation with anti-CCP levels. Meanwhile, miR-7 and ciRS-7 levels significantly correlated with the duration of RA (<em>r<sub>s</sub></em> = 0.32, <em>p</em> = 0.03; <em>r<sub>s</sub></em> = 0.4, <em>p</em> = 0.01, respectively), while the anti-CCP levels demonstrated a significant negative correlation with disease duration (<em>r<sub>s</sub></em>= −0.33, <em>p</em> = 0.03). miR-7 and ciRS-7 significantly distinguish cases from controls at cut-off values of < 0.81 and > 1.7, respectively (AUC = 0.98 and 0.99; <em>p</em> < 0.001), and their combination achieved 100 % diagnostic accuracy, with an AUC of 1 (<em>p</em> < 0.001). <em>Conclusions:</em> miR-7 and ciRS-7 have an inverse relationship, suggesting that ciRS-7 could modulate miR-7 regulatory functions. Both miR-7 and ciRS-7 could be used as diagnostic biomarkers for RA, and their combination could improve RA prediction.</div></div>\",\"PeriodicalId\":46152,\"journal\":{\"name\":\"Egyptian Rheumatologist\",\"volume\":\"47 2\",\"pages\":\"Pages 80-84\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-01-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Egyptian Rheumatologist\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1110116424001066\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"RHEUMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Rheumatologist","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1110116424001066","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"RHEUMATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景:包括miR-7和ciRS-7在内的非编码rna (ncRNAs)与许多自身免疫性疾病有关,但它们在类风湿关节炎(RA)中的作用在很大程度上尚未被探索。研究目的:通过比较miR-7和ciRS-7在患者和对照组中的表达模式,并将其与抗环瓜氨酸肽(anti-CCP)水平和临床表现相关联,评估miR-7和ciRS-7作为RA的诊断性生物标志物。患者和方法:纳入45例患者和45例对照。采用酶联免疫吸附试验(ELISA)评估血浆抗ccp水平,采用逆转录聚合酶链反应(RT-PCR)检测miR-7和ciRS-7表达水平。结果:所有病例均为女性,平均年龄39.6±10.2岁。病例的miR-7表达水平明显低于对照组(中位数,0.51 vs. 1.03;p & lt;0.001),而在病例中观察到ciRS-7显著升高(中位数,4.29 vs. 0.93;p & lt;0.001)。miR-7和ciRS-7与抗ccp水平均无显著相关性。miR-7、ciRS-7水平与RA病程显著相关(rs = 0.32, p = 0.03;rs= 0.4, p = 0.01),而抗ccp水平与病程呈显著负相关(rs= - 0.33, p = 0.03)。miR-7和ciRS-7在临界值<上显著区分病例与对照组;0.81和>;分别为1.7 (AUC = 0.98和0.99);p & lt;0.001),其组合达到100%的诊断准确率,AUC为1 (p <;0.001)。结论:miR-7与ciRS-7呈反比关系,表明ciRS-7可以调节miR-7的调控功能。miR-7和ciRS-7都可以作为RA的诊断性生物标志物,它们的结合可以提高RA的预测。
Expression patterns of ciRS-7 and miR-7 in peripheral blood mononuclear cells of rheumatoid arthritis patients
Background: Non-coding RNAs (ncRNAs), including miR-7 and ciRS-7, are implicated in many autoimmune diseases, but their roles in rheumatoid arthritis (RA) stay largely unexplored. Aim of the work: To assess miR-7 and ciRS-7 as diagnostic biomarkers for RA by comparing their expression patterns in patients vs. controls and correlating their profiles with anti-cyclic citrullinated peptide (anti-CCP) levels and clinical findings. Patients and methods: Forty-five patients and 45 controls were enrolled. Enzyme-linked immunosorbent assay (ELISA) was performed to assess plasma anti-CCP levels, and reverse transcription polymerase chain reaction (RT-PCR) was conducted to determine miR-7 and ciRS-7 expression levels. Results: All cases were female with a mean age of 39.6 ± 10.2 years. Cases exhibited significantly lower levels of miR-7 expression than control (median, 0.51 vs. 1.03; p < 0.001), while a significant elevation in ciRS-7 was observed in cases (median, 4.29 vs. 0.93; p < 0.001). Neither miR-7 nor ciRS-7 showed a significant correlation with anti-CCP levels. Meanwhile, miR-7 and ciRS-7 levels significantly correlated with the duration of RA (rs = 0.32, p = 0.03; rs = 0.4, p = 0.01, respectively), while the anti-CCP levels demonstrated a significant negative correlation with disease duration (rs= −0.33, p = 0.03). miR-7 and ciRS-7 significantly distinguish cases from controls at cut-off values of < 0.81 and > 1.7, respectively (AUC = 0.98 and 0.99; p < 0.001), and their combination achieved 100 % diagnostic accuracy, with an AUC of 1 (p < 0.001). Conclusions: miR-7 and ciRS-7 have an inverse relationship, suggesting that ciRS-7 could modulate miR-7 regulatory functions. Both miR-7 and ciRS-7 could be used as diagnostic biomarkers for RA, and their combination could improve RA prediction.