2023快速DNA多实验室研究结果- RapidINTEL Plus样品盒

Q3 Medicine

Forensic Science International: Reports Pub Date : 2024-12-01 DOI:10.1016/j.fsir.2024.100396

Erica L. Romsos , Lilliana I. Moreno , Douglas R. Hares

{"title":"2023快速DNA多实验室研究结果- RapidINTEL Plus样品盒","authors":"Erica L. Romsos , Lilliana I. Moreno , Douglas R. Hares","doi":"10.1016/j.fsir.2024.100396","DOIUrl":null,"url":null,"abstract":"<div><div>Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages.</div></div>","PeriodicalId":36331,"journal":{"name":"Forensic Science International: Reports","volume":"10 ","pages":"Article 100396"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Results of the 2023 rapid DNA multi-laboratory study – RapidINTEL Plus sample cartridge\",\"authors\":\"Erica L. Romsos , Lilliana I. Moreno , Douglas R. Hares\",\"doi\":\"10.1016/j.fsir.2024.100396\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages.</div></div>\",\"PeriodicalId\":36331,\"journal\":{\"name\":\"Forensic Science International: Reports\",\"volume\":\"10 \",\"pages\":\"Article 100396\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Forensic Science International: Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2665910724000458\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic Science International: Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2665910724000458","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

摘要

向六个外部实验室提供了相同的灵敏度和混合样本集，以评估最近对犯罪现场类型样本的快速DNA技术的改进。数据被送回联邦调查局和国家标准与技术研究所进行审查和分析。对所有样本进行人工解释（改进的快速DNA分析），以评估完整谱和20个组合DNA索引系统（CODIS）核心STR位点的基因分型成功，使用18个总细胞DNA输入量，范围为102.4 ng至0.025 ng。分析了两种总DNA输入的不同比例的两人和三人混合，并将观察到的等位基因百分比与预期的等位基因百分比进行了比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Results of the 2023 rapid DNA multi-laboratory study – RapidINTEL Plus sample cartridge

Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Forensic Science International: Reports Medicine-Pathology and Forensic Medicine

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

57 days