细胞角蛋白19：口腔舌鳞状细胞癌循环肿瘤细胞的潜在生物标志物

IF 0.4 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral and Maxillofacial Surgery Medicine and Pathology Pub Date : 2024-06-24 DOI:10.1016/j.ajoms.2024.06.006

Noble T. Baby , Shaji Thomas , R. Rejnish Kumar , Jayasree Kattoor , S. Kannan

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引用次数: 0

摘要

目的口腔舌鳞状细胞癌是口腔最常见的肿瘤之一。缺乏一个标志性的生物标志物来评估治疗反应，使得总生存率更低。细胞角蛋白是细胞骨架的中间纤维，被证明是检测不同癌症循环肿瘤细胞（CTC）的标志物。本研究旨在评估细胞角蛋白19 （CK19）作为口腔舌鳞状细胞癌（OTC）患者CTC标志物的潜力。方法采用实时聚合酶链反应（RT-PCR）技术检测CK19在OTC患者外周血单个核细胞（PBMC）中的表达规律，并与患者各项临床病理参数进行相关性分析。通过卡方检验评估各种临床病理参数与CK19表达之间的关系。结果CK19 mRNA水平与患者的临床分期（P = 0.021）和淋巴结状态（P = 0.011）显著相关。此外，CK19表达与患者的总生存率有显著相关性（P = 0.017）。结论外周血CK19 mRNA表达谱可用于CTCs监测，可作为OTC患者预后的生物标志物。

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Cytokeratin 19 a potential biomarker for circulating tumor cells in oral tongue squamous cell carcinoma

Objective

Oral tongue squamous cell carcinoma is one of the most common cancers in the oral cavity. The lack of a signature biomarker to assess the treatment response makes the overall survival rate still less. Cytokeratins are intermediate filaments of the cytoskeleton and shown to be used as a marker to detect circulating tumor cells (CTC) of different carcinomas. The present study aims to evaluate the potential of cytokeratin 19 (CK19) as a marker for CTC in oral tongue squamous cell carcinoma (OTC) patients.

Methods

The expression pattern of CK19 was assessed in peripheral blood mononuclear cells (PBMC) of OTC patients by real-time polymerase chain reaction (RT-PCR) and correlated to various clinico-pathological parameters of patients. The association between various clinico-pathological parameters and the expression of CK19 was evaluated by the Chi-Square Test.

Results

The results suggest that CK19 mRNA level is significantly associated with the clinical stage (P = 0.021) and nodal status (P = 0.011) of patients. Also, a significant association is observed with overall survival (P = 0.017) of the patients with CK19 expression.