瓜尔半乳甘露聚糖改善小鼠辐射诱导的肠道损伤

Darshini Shivamogga Mohan , Murali Badanthadka , Anushri Umesh , Bharath Basavapattana Rudresh , Manjunatha Bukkambudhi Krishnaswamy , Rashmi Kanugodu Vasappa , Sahayog Narayan Jamdar , Vidya Shimoga Muddappa
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引用次数: 0

摘要

瓜尔半乳甘露聚糖(GGM)是一种广泛应用于食品工业的水胶体,对其放射性防护的生物活性进行了评价。在体外淋巴细胞增殖研究中,GGM浓度为50 μg/ml时,血液淋巴细胞明显增殖。30天的生存研究表明,GGM预处理使7.5 Gy辐照小鼠的存活率提高到70%,而对照组的存活率仅为30%。第3天,与辐照对照组相比,经GGM预处理的IR小鼠组WBC计数维持正常。与辐照组相比,GGM预处理组肝脏血清酶水平降低,脾脏和胸腺重量指数及肠道抗氧化酶水平升高。第3天对回肠和空肠的组织病理学研究显示,与辐照对照组相比,GGM预处理组小鼠的隐窝和绒毛高度损伤较小。在空肠的差异基因表达中,共有43个基因出现差异表达,提示它们参与了半乳甘露聚糖处理对辐照小鼠的生物学反应。基因的差异表达表明,基因的上调与限制肠道和干细胞基因的损伤有关,这些基因参与细胞分化和功能。此外,在ggm预处理的IR组中,与凋亡和炎症相关的基因被显示下调。这些结果表明,GGM通过增加WBC计数、减轻急性肝损伤、维持脾脏和胸腺重量、增加抗氧化酶水平和改变基因表达来改善小鼠辐射损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Guar galactomannan ameliorates radiation-induced intestinal injury in mice
Guar galactomannan (GGM), a widely used hydrocolloid in the food industry, was evaluated for its biological activity with respect to radioprotection. In an in vitro lymphocyte proliferation study, GGM showed significant proliferation of blood lymphocytes at a concentration of 50 μg/ml. The survival study of 30 days showed that GGM pre-treatment improves the survival in irradiated (7.5 Gy) mice to 70% which otherwise is just 30% in the control irradiated group. On day 3, the WBC count was maintained in the GGM pretreated IR mice group compared to the irradiated control group. Serum enzyme levels in the liver of the GGM pretreated irradiated group were reduced, spleen and thymus weight indices and antioxidant enzymes were increased in the intestine of the GGM pretreated irradiated group compared to the irradiated group. Histopathological studies of ileum and jejunum on day 3 revealed that there was less damage in the crypt and height of villi in the GGM pretreated IR mice group compared to the irradiated control group. In the differential gene expression of jejunum, a total of 43 genes showed differential expressions, suggesting their involvement in the biological response to the effect of galactomannan treatment on irradiated mice. Differential expression of genes shows that upregulation of the genes is related to limiting the damage to the intestine and stem cell genes involved in cell differentiation and function. Moreover, genes associated with apoptosis and inflammation were shown to be downregulated in the GGM-pretreated IR group. These results suggest that GGM improves mice from radiation-induced injury by increasing WBC count, reducing acute liver damage, maintaining spleen and thymus weight, increasing the levels of antioxidant enzymes and altering gene expression.
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来源期刊
Bioactive Carbohydrates and Dietary Fibre
Bioactive Carbohydrates and Dietary Fibre Agricultural and Biological Sciences-Food Science
CiteScore
6.00
自引率
0.00%
发文量
38
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