Potential remineralizing effect of cuttlefish bone and eggshell Powder's on demineralized human enamel (an invitro study)

Dental treatment has been shifted towards more conservative approaches that focus on caries identification at early stage, remineralization of dental surfaces, and preservation of tooth structure. So that, newly safe alternative methods for teeth remineralization have to be created. There is minimal evidence to support the use of eggshell powder (ESP) for remineralizing enamel and Cuttlefish bone powder (CBP) as an alternative calcium source in bone replacement.The aim of this study is to evaluate the capacity of eggshell powder (ESP) and cuttlefish bone powder (CBP) solutions to remineralize early initiated demineralized lesions of enamel in permanent teeth. 40 extracted premolars were divided into four groups: Group1 (which received no treatment), Group2 (which was subjected todemineralizing solution), Group3 (which was subjected todemineralizing solution and then treated with CBP solution), and Group4 (which was subjected todemineralizing solution and then treated with ESPsolution). All groups were prepared forscanning electron microscope (SEM), X-ray microanalysis (EDAX), and microhardness evaluation. The demineralization process significantly changed the surface structure of the enamel, resulting in erosive lesions. CBP and ESP solutions both had a reparative effect, enhanced surface morphology, and increased Ca and P content and microhardness.