口腔鳞状细胞癌不同组织学变异中癌相关成纤维细胞的评估

Reshma Poothakulath Krishnan , Deepak Pandiar , Pratibha Ramani , Neha Kannan , Selvaraj Jayaraman
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引用次数: 0

摘要

癌症相关成纤维细胞是异质活化的成纤维细胞,是肿瘤微环境的核心组成部分。这些细胞通过激活各种信号通路和启动上皮间质转化来影响肿瘤的行为。CAFs在口腔鳞状细胞癌(OSCC)中有报道,然而,很少有研究评估CAFs在不同OSCC变体中的作用。本研究的目的是比较CAFs在常规OSCC和不同OSCC变体中的作用。材料与方法选取高分化、中分化、低分化OSCC各8例,疣状癌和疣状癌转化为OSCC各5例,梭形细胞癌、基底样OSCC和细胞分化明显的OSCC各3例,乳头状OSCC 2例,采用α-SMA免疫组化方法对其进行鉴定和评价。Kaplan Meier分析评估患者的总生存期。结果CAF免疫染色从乳头状鳞状细胞癌[平均- 1]到基底样鳞状细胞癌[1.6±0.5]到梭形细胞癌和鳞状细胞癌伴透明细胞改变[2.6±0.5],呈进行性升高(p = 0.00,有统计学意义)。CAF表达丰富的患者与CAF免疫组化染色少或局灶的患者相比,生存率较低。结论CAF免疫表达在PDSCCs、vc转化为SCC、透明细胞改变的OSCC和梭形细胞癌中均有升高。这突出了这些肿瘤的侵袭性。筛查这些肿瘤是否存在caf将有助于制定治疗计划,并有助于治疗计划和生存分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of cancer associated fibroblasts amongst different histological variants of oral squamous cell carcinoma

Background

Cancer associated fibroblasts are heterogenous activated fibroblasts that form the central component of the tumor microenvironment. These cells influence the behavior of the tumor by activating various signaling pathways and initiating epithelial mesenchymal transition. CAFs have been reported in oral squamous cell carcinomas (OSCC), however, very few studies have evaluated the role of CAFs in different variants of OSCC. The aim of this study was to compare the role of CAFs in conventional OSCC and different variants of OSCCs.

Materials and methods

Eight each of well differentiated, moderately differentiated and poorly differentiated OSCC, 5 each of verrucous carcinoma and verrucous carcinoma turning into OSCC, three each of spindle cell carcinoma, basaloid OSCC and OSCC with clear cell differentiation and 2 cases of papillary OSCC were retrieved, and immunohistochemistry was performed with α-SMA to identify and evaluate the CAFs. Kaplan Meier analysis was carried out to evaluate the overall survival of the patients.

Results

Progressive increase in the CAF immunostaining was noted from papillary squamous cell carcinoma [mean - 1] to basaloid squamous cell carcinoma [1.6 ± 0.5] to spindle cell carcinoma and squamous cell carcinoma with clear cell changes [2.6 ± 0.5] (p = 0.00 - statistically significant). Patients with abundant CAF expression showed lesser survival rate when compared to patients with scanty or focal CAF immunohistochemical staining.

Conclusion

Increased CAF immunoexpression was noted in PDSCCs, VCs turning into SCC, OSCC with clear cell changes and spindle cell carcinoma. This highlights the aggressive nature of these tumors. Screening these tumor for the presence of CAFs will help in treatment planning and will aid in treatment planning and survival analysis.
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