伊朗西北部地区乳腺癌患者MALAT1和BCYRN1 lncRNAs的表达

IF 0.5 Q4 GENETICS & HEREDITY

Human Gene Pub Date : 2025-02-01 DOI:10.1016/j.humgen.2024.201370

Alireza Ahmadi, Amin Moqadami, Mohammad Khalaj-Kondori, Mohammad Ali Hosseinpour Feizi

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引用次数: 0

摘要

在癌症组织中异常表达的lncrna可以潜在地作为癌症诊断、预后和治疗的生物标志物。本研究旨在评估MALAT1和BCYRN1在乳腺癌（BC）中的表达及其生物标志物潜力。方法收集70例乳腺癌患者癌旁组织和非癌旁组织配对标本，进行RNA提取和cDNA合成。采用qRT-PCR法检测MALAT1和BCYRN1 lncrna的表达。此外，我们还研究了MALAT1与BCYRN1表达水平的相关性。结果qRT-PCR结果显示，与BC患者边缘样本相比，肿瘤组织中MALAT1和BCYRN1水平明显升高。MALAT1表达与肿瘤分期极相关（p = 0.031）。ROC曲线分析表明BCYRN1可能被认为是BC的潜在生物标志物，而MALAT1在本研究中表现不佳。此外，MALAT1与BCYRN1表达水平之间存在统计学上不显著的正相关。结论MALAT1和BCYRN1 lncRNAs在乳腺癌组织中的表达水平较正常边缘明显上调。

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MALAT1 and BCYRN1 lncRNAs expression in patients with breast cancer from Northwest Iran

Introduction

Abnormally expressed lncRNAs in cancer tissues can potentially play as biomarkers for cancer diagnosis, prognosis, and treatment. This study aimed to assess the expression and biomarker potential of MALAT1 and BCYRN1 in breast cancer (BC).

Methods

Seventy paired samples of cancerous and non-cancerous adjacent tissues from breast cancer patients were collected and used for RNA extraction and cDNA synthesis. The expressions of MALAT1 and BCYRN1 lncRNAs were evaluated by qRT-PCR. In addition, the correlation between the expression levels of MALAT1 and BCYRN1 was investigated.

Results

The qRT-PCR results revealed that MALAT1 and BCYRN1 levels were considerably elevated in tumor tissues compared to the marginal samples of BC patients. Furthermore, MALAT1 expression was extremely correlated with tumor stages (p = 0.031). ROC curve analysis indicated that BCYRN1 might be considered a potential biomarker for BC, while MALAT1 showed poor results in this study. In addition, there was a statistically non-significant positive correlation between MALAT1 and BCYRN1 expression levels.

Conclusion

In conclusion, the expression levels of MALAT1 and BCYRN1 lncRNAs were significantly upregulated in breast cancer tissues compared to normal margins.

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来源期刊

Human Gene Biochemistry, Genetics and Molecular Biology (General), Genetics

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

54 days